SMURF2 Antibody (690638) [Alexa Fluor® 750]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 690638
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

SMURF2 Antibody (690638) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human SMURF2
  Met490-Glu748
  Accession # Q9HAU4
• Specificity: Detects human SMURF2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SMURF1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMURF2 Antibody (690638) [Alexa Fluor® 750]

• DKFZp686F0270
• E3 ubiquitin ligase SMURF2
• E3 ubiquitin-protein ligase SMURF2
• EC 6.3.2
• EC 6.3.2.-
• hSMURF2
• MGC138150
• SMAD specific E3 ubiquitin protein ligase 2
• SMAD ubiquitination regulatory factor 2
• SMAD-specific E3 ubiquitin-protein ligase 2
• SMURF2

Background

SMURF2 (SMAD ubiquitin regulatory factor-2) is a ~86 kDa, 748 amino acid (aa) cytoplasmic E3 ubiquitin ligase that negatively regulates TGF-beta signaling by promoting proteasomal degradation of some SMAD signaling molecules. It also influences cell cycle progression, peaking during mitosis and binding MAD2L1 and NEDD9/CASL. SMURF2 contains C2, WW1-3, and HECT domains. A potential 735 aa form lacks aa 19-31 (within the C2 domain), while a 507 aa form shows an alternate start site at aa 242 (between WW1 and WW2). Within the HECT domain region used as an immunogen, human SMURF2 shares 99% and 98% aa identity with mouse and rat SMURF2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9HAU4()