SMURF2 Antibody (690638) [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 690638
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

SMURF2 Antibody (690638) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human SMURF2
  Met490-Glu748
  Accession # Q9HAU4
• Specificity: Detects human SMURF2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human SMURF1 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMURF2 Antibody (690638) [Alexa Fluor® 488]

• DKFZp686F0270
• E3 ubiquitin ligase SMURF2
• E3 ubiquitin-protein ligase SMURF2
• EC 6.3.2
• EC 6.3.2.-
• hSMURF2
• MGC138150
• SMAD specific E3 ubiquitin protein ligase 2
• SMAD ubiquitination regulatory factor 2
• SMAD-specific E3 ubiquitin-protein ligase 2
• SMURF2

Background

SMURF2 (SMAD ubiquitin regulatory factor-2) is a ~86 kDa, 748 amino acid (aa) cytoplasmic E3 ubiquitin ligase that negatively regulates TGF-beta signaling by promoting proteasomal degradation of some SMAD signaling molecules. It also influences cell cycle progression, peaking during mitosis and binding MAD2L1 and NEDD9/CASL. SMURF2 contains C2, WW1-3, and HECT domains. A potential 735 aa form lacks aa 19-31 (within the C2 domain), while a 507 aa form shows an alternate start site at aa 242 (between WW1 and WW2). Within the HECT domain region used as an immunogen, human SMURF2 shares 99% and 98% aa identity with mouse and rat SMURF2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.