SMNDC1 Antibody

Images

 
Western Blot: SMNDC1 Antibody [NBP1-47302] - Whole cell lysates from ovarian surface epithial cells (OSE), ovarian cancer cell line A2780, breast cancer cell line MCF7. Image from verified customer review.
Western Blot: SMNDC1 Antibody [NBP1-47302] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

SMNDC1 Antibody Summary

Immunogen
The immunogen for this product maps to a region between residue 188 and 238 of human Splicing Factor 30 using the numbering given in entry NP_005862.1 (GeneID 10285).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMNDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47302 in the following applications:

Publications
Read Publication using
NBP1-47302 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMNDC1 Antibody

  • SMNRSMN-related protein
  • SPF30splicing factor 30, survival of motor neuron-related
  • survival motor neuron domain containing 1,30 kDa splicing factor SMNrp
  • Survival motor neuron domain-containing protein 1
  • survival of motor neuron-related-splicing factor 30

Background

This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for SMNDC1 Antibody (NBP1-47302)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Review for SMNDC1 Antibody (NBP1-47302) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-47302:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SMNDC1 NBP1-47302
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reviewed by:
Prabir Chakraborty
WB Human 06/02/2014
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Summary

ApplicationWestern Blot
Sample TestedWHOLE CELL LYSATES FROM OVARIAN SURFACE EPITHIAL CELLS(OSE), OVARIAN CANCER CELL LINE A2780, BREAST CANCER CELL LINE MCF7
SpeciesHuman
LotA1
CommentsDETECTS A SPECIFIC BAND BELOW 37KD.

Blocking

Blocking Details3% BSA IN TBST FOR 1H AT RT.

Primary Anitbody

Dilution Ratio1:2000 IN 3% BSA/TBST, OVERNIGHT AT 4*C.

Secondary Antibody

Secondary DescriptionGOAT ANTI-RABBIT IgG-HRP
Secondary Manufacturer Cat#sc-2301
Secondary Concentration1:10000 IN TBST

Details

Detection NotesECL USING CLARITY WESTERN ECL SUBSTRATE, DETECTION TIME 10 SECONDS.

Comments

CommentsDETECTS A SPECIFIC BAND BELOW 37KD.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SMNDC1 Antibody (NBP1-47302) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SMNDC1 Antibody (NBP1-47302)

Discover related pathways, diseases and genes to SMNDC1 Antibody (NBP1-47302). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SMNDC1 Antibody (NBP1-47302)

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PTMs for SMNDC1 Antibody (NBP1-47302)

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Research Areas for SMNDC1 Antibody (NBP1-47302)

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Blogs on SMNDC1

There are no specific blogs for SMNDC1, but you can read our latest blog posts.

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Contact Information

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Calculators

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Recent Reviews

5
5
1
4
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1
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Prabir Chakraborty
06/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SMNDC1
Entrez