Genetic Strategies: Western Blot: DIS3 Antibody [H00022894-B01P] - analysis of DIS3 in HeLa whole cell extracts treated with (1) si-Control and (2) DIS3 siRNA using anti-DIS3 antibody. Image from verified ...read more
Western Blot: DIS3 Antibody [H00022894-B01P] - Analysis of DIS3 expression in transfected 293T cell line by DIS3 polyclonal antibody. Lane 1: KIAA1008 transfected lysate(105.38 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DIS3 Antibody [H00022894-B01P] - Analysis of purified antibody to KIAA1008 on HeLa cell. (antibody concentration 10 ug/ml)
1. After Western blotting, membrane was blocked for 30 minutes in 5% milk dissolved in PBS containing 0.1% Tween 20 (PBST).2. Membrane was blotted with primary antibody diluted in PBST at 4C overnight.
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