DIS3 Antibody

Western Blot: DIS3 Antibody [H00022894-B01P] - analysis of DIS3 in HeLa whole cell extracts treated with (1) si-Control and (2) DIS3 siRNA using anti-DIS3 antibody. Image from verified customer review.

Western Blot: DIS3 Antibody [H00022894-B01P] - Analysis of DIS3 expression in transfected 293T cell line by DIS3 polyclonal antibody. Lane 1: KIAA1008 transfected lysate(105.38 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: DIS3 Antibody [H00022894-B01P] - Analysis of purified antibody to KIAA1008 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu, Mu, Ch
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated

DIS3 Antibody Summary

• Immunogen: KIAA1008 (AAH56143.1, 1 a.a. - 958 a.a.) full-length human protein. MLKSKTFLKKTRAGGVMKIVREHYLRDDIGCGAPGCAACGGAHEGPALEPQPQDPASSVCPQPHYLLPDTNVLLHQIDVLEDPAIRNVIVLQTVLQEVRNRSAPVYKRIRDVTNNQEKHFYTFTNEHHRETYVEQEQGENANDRNDRAIRVAAKWYNEHLKKMSADNQLQVIFITNDRRNKEKAIEEGIPAFTCEEYVKSLTANPELIDRLACLSEEGNEIESGKIIFSEHLPLSKLQQGIKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGLKHLNRAVHEDIVAVELLPKSQWVAPSSVVLHDEGQNEEDVEKEEERERMLKTAVSEKMLKPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASTLEGRRIIVAIDGWPRNSRYPNGHFVRNLGDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEKDMKNREDLRHLCICSVDPPGCTDIDDALHCRELENGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLKCDVDRLAFSCIWEMNHNAEILKTKFTKSVINSKASLTYAEAQLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: DIS3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is useful for Western Blot, Immunofluorescence

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DIS3 Antibody

• 2810028N01Rik
• DIS3 mitotic control homolog (S. cerevisiae)
• dis3p
• DKFZp667L1817
• EC 3.1.13
• EC 3.1.13.-
• EC 3.1.26.-
• EXOSC11
• exosome complex exonuclease RRP44
• exosome component 11
• FLJ10484
• KIAA1008RP11-342J4.3
• MGC33035
• mitotic control protein dis3 homolog
• Protein DIS3 homolog
• Ribosomal RNA-processing protein 44
• RRP44bA555G22.1

Background

DIS3 is a component of the exosome 3'->5' exoribonuclease complex. Required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. Also associated with the GTPase Ran. Has a 3'-5' exonuclease activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DIS3 Antibody (H00022894-B01P)(3)

Publications using H00022894-B01P

• Ogami K, Richard P, Chen Y et al. An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. Gens & Dev. 2017-07-21 [PMID: 28733371]
• Hsin JP, Li W, Hoque M et al. RNAP II CTD tyrosine 1 performs diverse functions in vertebrate cells. Elife (Cambridge) 2014-06-04 [PMID: 24842995] (WB, Chicken)
  
  Details:
  EXOSC3 antibody used for WB on lysates of 26r (DT40 cells derived Rpb1 derivative containing a CTD with 26 YSPTSPS repeats) and 25F+Y cells (Rpb1-Y1F derivative in which only a single F, in the C terminal-most heptad, was changed back to Y). WB data shown in Supplement Figure 4.
• Xie M, Li M, Vilborg A et al. Mammalian 5'-Capped MicroRNA Precursors that Generate a Single MicroRNA. Cell. 2013-12-19 [PMID: 24360278]

Reviews for DIS3 Antibody (H00022894-B01P) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Other.

reviewed by: Koichi Ogami WB Human 05/05/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: D9091

reviewed by: Verified Customer WB Other 10/18/2013

Summary

• Application: Western Blot
• Species: Other

Bioinformatics

• Gene Symbol: DIS3
• Uniprot:
  • AAH56143.1(Human)