Smek1 Antibody - BSA Free

Western Blot: Smek1 Antibody [NBP2-94709] - Analysis of extracts of various cell lines, using Smek1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Immunohistochemistry-Paraffin: Smek1 Antibody [NBP2-94709] - Immunohistochemistry of paraffin-embedded mouse kidney using Smek1 Rabbit pAb (NBP2-94709) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Smek1 Antibody [NBP2-94709] - Immunohistochemistry of paraffin-embedded rat ovary using Smek1 Rabbit pAb (NBP2-94709) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: Smek1 Antibody [NBP2-94709] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Smek1 Rabbit pAb (NBP2-94709) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Smek1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Smek1 Antibody - BSA Free (NBP2-94709) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 395-594 of human SMEK1 (NP_001271210.1).VQTFKGLKLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDMEDGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQSPSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQTAAITTKGGLVGLVDYPDDDEDDDEDEDKEDTLPLSKKAKFDS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP4R3A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Smek1 Antibody - BSA Free

• FLFL1
• FLJ20707
• KIAA2010MSTP033
• PP4R3A
• PPP4R3A
• serine/threonine-protein phosphatase 4 regulatory subunit 3A
• SMEK homolog 1
• SMEK homolog 1, suppressor of mek1 (Dictyostelium)
• smk1
• smk-1

Background

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP4R3A