SMEK2 Antibody

Images

 
Western Blot: SMEK2 Antibody [NBP1-83882] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: SMEK2 Antibody [NBP1-83882] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
Immunohistochemistry-Paraffin: SMEK2 Antibody [NBP1-83882] - Staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Western Blot: SMEK2 Antibody [NBP1-83882] - Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line EFO-21. Lane 4: Human cell line A-431

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SMEK2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:GQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDEEEEGKAVMPPLE
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMEK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
SMEK2 Protein (NBP1-83882PEP)
Publications
Read Publication using NBP1-83882.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SMEK2 Antibody

  • FLFL2
  • FLJ31474
  • KIAA1387serine/threonine-protein phosphatase 4 regulatory subunit 3B
  • PP4R3B
  • PPP4R3B
  • PSY2
  • SMEK homolog 2
  • SMEK homolog 2, suppressor of mek1 (Dictyostelium)
  • smk1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMEK2 Antibody (NBP1-83882)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMEK2 Antibody (NBP1-83882) (0)

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Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to SMEK2 Antibody (NBP1-83882). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Contact Information

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Calculators

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Bioinformatics

Gene Symbol SMEK2