PP4/PPP4C Antibody (501517)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PP4 Catalytic Subunit Monoclonal Antibody (Catalog # MAB5074) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP4 Catalytic Subunit at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 501517
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PP4/PPP4C Antibody (501517) Summary

• Immunogen: E. coli-derived recombinant human PP4 Catalytic Subunit
  Met1-Leu307
  Accession # P60510
• Specificity: Detects human, mouse, and rat PP4 Catalytic Subunit in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP4C
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PP4/PPP4C Antibody (501517)

• EC 3.1.3.16
• PP4
• PP4C
• PP4protein phosphatase X, catalytic subunit
• PPH3
• PPP4C
• PPX
• PP-X
• PPXPPH3
• protein phosphatase 4 (formerly X), catalytic subunit
• protein phosphatase 4, catalytic subunit
• Protein phosphatase X

Background

The catalytic subunit of Protein Phosphatase 4 (PP4), also called PPX, is a serine/threonine protein phosphatase with about 65% homology to PP2A. Although PP4 has the same heterotrimeric subunit structure as PP2A and can use many of the PP2A regulatory subunits, PP4 also has its own unique scaffolding and regulatory subunits. PP4 is largely found in the centrosome of the nucleus where it may play a role in modulating transcription factor phosphorylation by JNK. In Drosophila, cmm mutants have low levels of PP4 and are unable to complete mitosis due to a deficit in microtubule assembly.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP4/PPP4C Antibody (MAB5074)(2)

Publications using MAB5074

• S Ohba, TA Johannesse, K Chatla, X Yang, RO Pieper, J Mukherjee Phosphoglycerate Mutase 1 Activates DNA Damage Repair via Regulation of WIP1 Activity Cell Rep, 2020-04-14;31(2):107518. 2020-04-14 [PMID: 32294440]
• Lai S, O'Callaghan B, Zoghbi HY 14-3-3 Binding to ataxin-1(ATXN1) regulates its dephosphorylation at Ser-776 and transport to the nucleus. J. Biol. Chem., 2011-08-11;286(40):34606-16. 2011-08-11 [PMID: 21835928] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PPP4C
• Uniprot:
  • P60510()