SMCHD1 Antibody (CL4270)

Images

 
Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP2-59046] - Staining of human skeletal muscle shows no positivity as expected (negative control).
Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP2-59046] - Staining of human testis shows moderate to strong nuclear positivity in seminiferous tubules cells.
Immunohistochemistry: SMCHD1 Antibody [NBP2-59046] - Staining of human small intestine shows strong nuclear immunoreactivity in glandular cells and in connective tissue cells.
Immunohistochemistry: SMCHD1 Antibody [NBP2-59046] - Staining of human placenta shows strong nuclear immunoreactivity in trophoblast.
Immunohistochemistry: SMCHD1 Antibody [NBP2-59046] - Staining of human lymph node shows weak to moderate nuclear immunoreactivity in lymphoid cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
CL4270
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SMCHD1 Antibody (CL4270) Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:DNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKED
Specificity
Specificity of human SMCHD1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (97%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMCHD1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SMCHD1 Antibody (CL4270)

  • DKFZp686O0631
  • KIAA0650
  • structural maintenance of chromosomes flexible hinge domain containing 1
  • structural maintenance of chromosomes flexible hinge domain-containing protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SMCHD1