DUX4 Antibody (P4H2)

Images

 
Western Blot: DUX4 Antibody (P4H2) [NBP1-49552] - Total protein from human testis tissue and human K562, HeLa and HepG2 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: DUX4 Antibody (P4H2) [NBP1-49552] - Detection of human DUX4 on C2C12 mouse myoblasts transfected with pCS2-DUX4. Cells were counterstained with DAPI for nuclei.
Immunohistochemistry: DUX4 Antibody (P4H2) [NBP1-49552] - Analysis of DUX4 on human testis
Western Blot: DUX4 Antibody (P4H2) [NBP1-49552] - Analysis of DUX4 expression in 1) human testis and 2) DUX4 protein lysates.
Simple Western: DUX4 Antibody (P4H2) [NBP1-49552] - Simple Western lane view shows a specific band for DUX4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
P4H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

DUX4 Antibody (P4H2) Summary

Immunogen
Synthetic peptide corresponding to the C-terminus of human DUX4. [Swiss-Prot# Q9UBX2]
Localization
Nucleus. Note: Actively transported through the nuclear pore complex (NPC).
Specificity
This antibody is specific for DUX4 and does not recognize DUX4c.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DUX4
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0ug/ml
  • Simple Western 1:10
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Frozen 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
This DUX4 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen sections, Immunohistochemistry paraffin embedded sections and Western Blot. In Western blot, a band can be seen at approx. 52 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-49552 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for DUX4 Antibody (P4H2)

  • double homeobox 4
  • double homeobox protein 10
  • double homeobox protein 4
  • double homeobox protein 4/10
  • double homeobox protein DUX10
  • DUX10
  • DUX4
  • DUX4L1

Background

DUX4 (also known as Double homeobox 4) is the leading candidate causative gene for facioscapulohumeral dystrophy (FSHD), a degenerative skeletal muscle disease and one of the most common muscular dystrophies. FSHD is caused by the deletion of a subset of D4Z4 macrosatellite repeats on chromosome 4. Each repeat contains a retrogene encoding the double-homeobox factor DUX4. DUX4 expression is epigenetically suppressed in differentiated tissues and the residual DUX4 transcripts are spliced to remove the carboxyterminal domain that has been associated with cell toxicity. In FSHD individuals, the expression of the full-length DUX4 transcript is not completely suppressed in skeletal muscle, and possibly other differentiated tissues, and results in a small percentage of cells expressing relatively abundant amounts of the full-length DUX4 mRNA and protein. [Snider et al. (2010) PLoS Genetics.6(10): e1001181]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUX4 Antibody (NBP1-49552)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA, ICC/IF, WB.


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ELISA
(1)
ICC/IF
(2)
WB
(2)
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Product General Protocols

View specific protocols for DUX4 Antibody (NBP1-49552): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DUX4 Antibody (NBP1-49552) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49552AF405
Alexa Fluor 488 Labeled NBP1-49552AF488
Alexa Fluor 647 Labeled NBP1-49552AF647
Alexa Fluor 700 Labeled NBP1-49552AF700
Biotin Labeled NBP1-49552B
DyLight 350 Labeled NBP1-49552UV
DyLight 405 Labeled NBP1-49552V
DyLight 488 Labeled NBP1-49552G
DyLight 550 Labeled NBP1-49552R
DyLight 650 Labeled NBP1-49552C
DyLight 680 Labeled NBP1-49552FR
DyLight 755 Labeled NBP1-49552IR
FITC Labeled NBP1-49552F
HRP Labeled NBP1-49552H

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Bioinformatics Tool for DUX4 Antibody (NBP1-49552)

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Bioinformatics

Gene Symbol DUX4
Entrez
Uniprot