| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82978-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44088 | |||
| BSA Free | NBP2-59045-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44089 | |||
| BSA Free | NBP2-59046-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59045 | |||
| BSA Free | NBP2-59045-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38399-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22190-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38399-100ul | |||
| BSA Free | NBP3-38399-20ul | |||
![Western Blot: SMCHD1 Antibody [NBP1-82978] - Analysis in human cell lines U2OS and MCF-7 using Anti-SMCHD1 antibody. Corresponding SMCHD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Western-Blot-NBP1-82978-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SMCHD1 Antibody [NBP1-82978] - Analysis in human cell lines U2OS and MCF-7 using Anti-SMCHD1 antibody. Corresponding SMCHD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody [NBP1-82978] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82978-img0016.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978] - Staining of human skeletal muscle shows very weak nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82978-img0020.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978] - Staining of human tonsil shows moderate nuclear positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82978-img0017.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978] - Staining of human testis shows moderate nuclear positivity in cells in seminiferuous ducts.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82978-img0018.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-82978] - Staining of human rectum shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-82978-img0019.jpg)
![Simple Western: SMCHD1 Antibody [NBP1-82978] - Electropherogram image(s) of corresponding Simple Western lane view. SMCHD1 antibody was used at 1:40 dilution on RT-4 lysates(s).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Simple-Western-NBP1-82978-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of U-2 OS cells using the Anti-SMCHD1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4265-Immunocytochemistry-Immunofluorescence-NBP2-59045-img0007.jpg "Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of U-2 OS cells using the Anti-SMCHD1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of human cerebellum shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4265-Immunohistochemistry-Paraffin-NBP2-59045-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of A-431 cells using the Anti-SMCHD1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4265-Immunocytochemistry-Immunofluorescence-NBP2-59045-img0006.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of human fallopian tube shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4265-Immunohistochemistry-Paraffin-NBP2-59045-img0001.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of human endometrium shows nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4265-Immunohistochemistry-Paraffin-NBP2-59045-img0002.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of human breast cancer shows moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4265-Immunohistochemistry-Paraffin-NBP2-59045-img0003.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4265) [NBP2-59045] - Staining of human stomach shows nuclear immunoreactivity in glandular cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4265-Immunohistochemistry-Paraffin-NBP2-59045-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of U-2 OS cells using the Anti-SMCHD1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4270-Immunocytochemistry-Immunofluorescence-NBP2-59046-img0009.jpg "Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of U-2 OS cells using the Anti-SMCHD1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human skeletal muscle shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4270-Immunohistochemistry-Paraffin-NBP2-59046-img0005.jpg)
![Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human testis shows moderate to strong nuclear positivity in seminiferous tubules cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4270-Immunohistochemistry-Paraffin-NBP2-59046-img0003.jpg)
![Immunohistochemistry: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human small intestine shows strong nuclear immunoreactivity in glandular cells and in connective tissue cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4270-Immunohistochemistry-NBP2-59046-img0006.jpg)
![Immunohistochemistry: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human placenta shows strong nuclear immunoreactivity in trophoblast.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4270-Immunohistochemistry-NBP2-59046-img0007.jpg)
![Immunohistochemistry: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human lymph node shows weak to moderate nuclear immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/SMCHD1-Antibody-CL4270-Immunohistochemistry-NBP2-59046-img0008.jpg)
![Western Blot: SMCHD1 Antibody [NBP1-49968] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Western-Blot-NBP1-49968-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMCHD1 Antibody [NBP1-49968] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-49968] - Sample: FFPE section of human testicular seminoma (left) and non-small cell lung cancer (right). Antibody: Affinity purified rabbit anti- SmcHD1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-NBP1-49968-img0004.jpg)
![Western Blot: SMCHD1 Antibody [NBP1-49969] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Western-Blot-NBP1-49969-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMCHD1 Antibody [NBP1-49969] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: SMCHD1 Antibody [NBP1-49969] - Sample: FFPE section of human non-small cell lung cancer (left) and testicular seminoma (right). Antibody: Affinity purified rabbit anti- SmcHD1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-NBP1-49969-img0002.jpg)
![Western Blot: SMCHD1 Antibody [NBP3-38399] - Western blot analysis of various lysates using SMCHD1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38399_rabbit-smchd1-pab-101220241155280.jpg "Western Blot: SMCHD1 Antibody [NBP3-38399] - Western blot analysis of various lysates using SMCHD1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: SMCHD1 Antibody [NBP3-38399] - Immunofluorescence analysis of MCF-7 cells using SMCHD1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38399_rabbit-smchd1-pab-101220241252868.jpg)
![Western Blot: SMCHD1 Antibody (JE34-29) [NBP3-32960] - Western blot analysis of SMCHD1 on different lysates with Rabbit anti-SMCHD1 antibody (NBP3-32960) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: U-2 OS cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 226 kDa Observed band size: 226 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32960) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32960_rabbit-smchd1-mab-je34-29-28820241249343.jpg "Western Blot: SMCHD1 Antibody (JE34-29) [NBP3-32960] - Western blot analysis of SMCHD1 on different lysates with Rabbit anti-SMCHD1 antibody (NBP3-32960) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: U-87 MG cell lysate Lane 5: U-2 OS cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 226 kDa Observed band size: 226 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32960) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SMCHD1 Antibody (JE34-29) [NBP3-32960] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SMCHD1 antibody (NBP3-32960) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32960) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32960_rabbit-smchd1-mab-je34-29-298202414333616.jpg)
![Western Blot: SMCHD1 Antibody (SR1339) [NBP3-22190] - Western blot analysis using NBP3-22190 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22190_rabbit-smchd1-mab-sr1339-57202316582190.jpg "Western Blot: SMCHD1 Antibody (SR1339) [NBP3-22190] - Western blot analysis using NBP3-22190 in 293T cell lysate.")