SMC5 Antibody - BSA Free

Western Blot: SMC5 Antibody [NBP2-20419] - Sample (30 ug of whole cell lysate) A: NT2D1 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: SMC5 Antibody [NBP2-20419] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (NBP2-20419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SMC5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMC5 Antibody - BSA Free (NBP2-20419) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SMC5. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 129 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SMC5 Antibody - BSA Free

• hSMC5
• KIAA0594SMC5 structural maintenance of chromosomes 5-like 1 (yeast)
• SMC protein 5
• SMC-5
• SMC5L1SMC5 structural maintenance of chromosomes 5-like 1
• structural maintenance of chromosomes 5
• structural maintenance of chromosomes protein 5

Background

Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for SMC5 Antibody (NBP2-20419). (Showing 1 - 1 of 1 FAQ).

1. What dilution should be used for immunoflourescence?
   • Unfortunately, our product NBP2-20419 has only been validated in western blot, so we do not have a recommended dilution for IF. If you would like to try this product in IF with cells or tissue, you would qualify for our Innovator's Reward Program. You would just need to submit your results (positive or negative) as a review on the product and we will send you a credit for the purchase price of the antibody. You can find details of this program here: Innovators Reward Program

Bioinformatics

• Gene Symbol: SMC5
• Uniprot:
  • Q8IY18(Human)