SMC1 Antibody (5B6)

Images

 
Western Blot: SMC1 Antibody (5B6) [NBP1-51596] - Western blot analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: SMC1 Antibody (5B6) [NBP1-51596] - Immunofluorescence analysis of NIH3T3 cells using SMC1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 ...read more
Immunohistochemistry-Paraffin: SMC1 Antibody (5B6) [NBP1-51596] - Immunohistochemical analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.
Flow Cytometry: SMC1 Antibody (5B6) [NBP1-51596] - Flow cytometric analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
5B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SMC1 Antibody (5B6) Summary

Immunogen
Purified recombinant fragment of human SMC1 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SMC1A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:1000
  • ELISA 1:10000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for SMC1 Antibody (5B6)

  • CDLS2
  • DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
  • KIAA0178DKFZp686L19178
  • SB1.8
  • SB1.8MGC138332
  • segregation of mitotic chromosomes 1
  • SMC protein 1A
  • SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
  • SMC1 structural maintenance of chromosomes 1-like 1
  • SMC1
  • SMC1A
  • SMC-1A
  • SMC-1-alpha
  • SMC1L1
  • SMC1SMC1alpha
  • Smcb
  • structural maintenance of chromosomes 1A
  • structural maintenance of chromosomes protein 1A

Background

SMC1L1/SMC1 (structural maintenance of chromosome 1-like 1 (yeast)) is a 145-160 kD heterodimeric protein that is a member of the SMC family. This nuclear protein dissociates from chromosomes before mitosis but remains associated with kinetochore during mitosis. SMC1L1/SMC1 is located on the X-chromosome and is involved in mitotic chromosome dynamics. The SMC family includes SMC3, SMC1L1, and SMC1L2 which form part of the cohesion multiprotein complex. This protein can also form heterodimers consisting of SMC1L1/SMC3 or SMCL2/SMC3 and may also be involved in DNA repair. SMC1L1/SMC1 is phosphorylated by ATM after DNA damage; phosphorylation is also required for sister chromatid cohesion and correct segregation of chromosomes during cell division. SMC1L1/SMC1 associates with BRCA1, and SYCP2, and interacts directly with SMC3 in the cohesion complex (linked by RAD21 complexed to one STAG protein). The Poly6096 antibody recognizes the N-terminal region of human and mouse SMC1L1/SMC1 and has been shown to be useful for Western blotting and immunofluorescence staining. BRCA1, SYCP2, interacts directly with SMC3 in the cohesion complex (linked by RAD21 complexed to one STAG protein)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMC1 Antibody (NBP1-51596). (Showing 1 - 2 of 2 FAQ).

  1. I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
    • My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
  2. My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
    • We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC1A
Entrez