SMARCAD1 Antibody

Western Blot: SMARCAD1 Antibody [NB100-79835] - Whole cell lysate (50 ug) from MDA-MB-231,MCF-7, HEK293T, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCAD1/ETL1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescencewith an exposure time of 3 minutes.

Western Blot: SMARCAD1 Antibody [NB100-79835] - Detection of Human and Mouse SMARCAD1/ETL1 on HeLa whole cell lysate using NB100-79835. SMARCAD1/ETL1 was also IPed by rabbit anti- SMARCAD1/ETL1 antibody NB100-79834.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SMARCAD1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 976 and 1026 of human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 (enhancer trap locus 1) using the numbering given in entry NP_064544.1 (GeneID 56916).
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCAD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Control:

  SMARCAD1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMARCAD1 Antibody

• ATP-dependent helicase 1
• DKFZP762K2015
• EC 3.6.1
• EC 3.6.4.12
• HEL1
• hHEL1
• KIAA1122ETL1DKFZp762K2015
• subfamily a, containing DEAD/H box 1
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A containing DEAD/H box 1
• SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin

Background

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. SMARCAD1/ETL1 is a unique member of this family in that it contains two DEAD/H box motifs, and thus is also a member of the DEAD/H-box-containing helicase superfamily. The chromosomal position of SMARCAD1 has been mapped, and it resides in a region rich in breakpoints and deletions associated with several human diseases. It is suggested to play a role in genetic instability and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SMARCAD1 Antibody (NB100-79835)(3)

Publications using NB100-79835

• Oh JM, Kang Y, Park J et al. MSH2-MSH3 promotes DNA end resection during homologous recombination and blocks polymerase theta-mediated end-joining through interaction with SMARCAD1 and EXO1 Nucleic Acids Research 2023-06-23 [PMID: 37140056] (Western Blot, Human)
• Horikoshi N, Sharma D, Leonard F et al. Pre-existing H4K16ac levels in euchromatin drive DNA repair by homologous recombination in S-phase Commun Biol 2019-07-05 [PMID: 31286070] (Human)
• Chakraborty S, Pandita RK, Hambarde S et al. SMARCAD1 Phosphorylation and Ubiquitination Are Required for Resection during DNA Double-Strand Break Repair. iScience. 2018-04-27 [PMID: 29888761] (WB, Human)

Bioinformatics

• Gene Symbol: SMARCAD1
• Entrez:
  • 56916(Human)
• Uniprot:
  • Q9H4L7(Human)