RENT1/UPF1/hUPF1 Antibody

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Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-RENT1 antibody used for ...read more
Western Blot: RENT1/UPF1/hUPF1 Antibody [NB100-368] - Whole cell lysate (30 ug) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody used at 1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RENT1/UPF1/hUPF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 250 and 300 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UPF1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1
Application Notes
By Western blot, this antibody recognizes a band at ~145 kDa, representing human RENT1. Suggested working dilutions: * Western Blot - 1:1,000-1:2,500 (ECL) Immunoprecipitation - ND Immunohistochemistry - ND *The investigator should determine the optimal working dilution for a specific application.
Publications
Read Publications using NB100-368.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RENT1/UPF1/hUPF1 Antibody

  • ATP-dependent helicase RENT1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ43809
  • HUPF1
  • KIAA0221FLJ46894
  • Nonsense mRNA reducing factor 1
  • NORF1
  • NORF1delta helicase
  • pNORF1
  • regulator of nonsense transcripts 1
  • RENT1
  • RENT1UP Frameshift 1
  • UPF1 regulator of nonsense transcripts homolog (yeast)
  • UPF1
  • up-frameshift mutation 1 homolog
  • Up-frameshift suppressor 1 homolog
  • yeast Upf1p homolog

Background

RENT1 (regulator of nonsense transcripts 1) is a group 1 RNA helicase, RNA-binding protein whose RNA-binding activity is modulated by ATP. It enters the nucleus where it influences early events in mRNA biogenesis. It functions in NMD (non-sense-mediated mRNA decay) in mammalian cells, ridding cells of aberrant mRNAs containing premature termination codons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RENT1/UPF1/hUPF1 Antibody (NB100-368)(9)


Showing Publications 1 - 9 of 9.
Publications using NB100-368 Applications Species
de Turris V, Nicholson P, Orozco RZ et al. Cotranscriptional effect of a premature termination codon revealed by live-cell imaging. RNA 2011 Dec [PMID: 22028363]
Peixeiro I, Inacio a, Barbosa C et al. Interaction of PABPC1 with the translation initiation complex is critical to the NMD resistance of AUG-proximal nonsense mutations. Nucleic Acids Res 2012 Feb [PMID: 21989405]
Boudoukha S, Cuvellier S, Polesskaya A et al. Role of the RNA-binding protein IMP-2 in muscle cell motility. Mol Cell Biol 2010 Dec [PMID: 20956565]
Valacca C, Bonomi S, Buratti E et al. Sam68 regulates EMT through alternative splicing-activated nonsense-mediated mRNA decay of the SF2/ASF proto-oncogene. J Cell Biol 2010 Oct [PMID: 20876280]
Wittkopp N, Huntzinger E, Weiler C et al. Nonsense-mediated mRNA decay effectors are essential for zebrafish embryonic development and survival. Mol Cell Biol 2009 Jul [PMID: 19414594]
Muller B, Blackburn J, Feijoo C et al. DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication. J Cell Biol 2007 Dec [PMID: 18158334]
Boutz PL, Stoilov P, Li Q et al. A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neurons. Genes Dev 2007 Jul [PMID: 17606642]
Ni JZ, Grate L, Donohue JP et al. Ultraconserved elements are associated with homeostatic control of splicing regulators by alternative splicing and nonsense-mediated decay. Genes Dev 2007 Mar [PMID: 17369403]
Silva AL, Pereira FJ, Morgado A et al. The canonical UPF1-dependent nonsense-mediated mRNA decay is inhibited in transcripts carrying a short open reading frame independent of sequence context. RNA 2006 Dec [PMID: 17077274]

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Bioinformatics

Gene Symbol UPF1
Entrez
Uniprot