SMARCAD1 Antibody - BSA Free

Immunoprecipitation: SMARCAD1 Antibody [NB100-79834] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCAD1/ETL1 antibody NB100-79834 used for IP at 3 mcg/mg lysate. SMARCAD1/ETL1 was also immunoprecipitated by rabbit anti-SMARCAD1/ETL1 antibody NB10-79835, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu, Bv
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SMARCAD1 Antibody - BSA Free Summary

• Immunogen: The immunogenfor this product falls between residues 1 and 50 of human SWI/SNFrelated matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 (enhancer trap locus 1) using the numbering given in entry NP_064544.1 (GeneID 56916).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCAD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
• Control:

  SMARCAD1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMARCAD1 Antibody - BSA Free

• ATP-dependent helicase 1
• DKFZP762K2015
• EC 3.6.1
• EC 3.6.4.12
• HEL1
• hHEL1
• KIAA1122ETL1DKFZp762K2015
• subfamily a, containing DEAD/H box 1
• SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A containing DEAD/H box 1
• SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin

Background

The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. SMARCAD1/ETL1 is a unique member of this family in that it contains two DEAD/H box motifs, and thus is also a member of the DEAD/H-box-containing helicase superfamily. The chromosomal position of SMARCAD1 has been mapped, and it resides in a region rich in breakpoints and deletions associated with several human diseases. It is suggested to play a role in genetic instability and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMARCAD1
• Entrez:
  • 56916(Human)
• Uniprot:
  • NP_064544.1(Human)