Smad9 Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu
• Applications: IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

Smad9 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Smad9
  Ala158-Ser223
  Accession # O15198
• Specificity: Detects human Smad9 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Smad1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad9 Antibody [Alexa Fluor® 488]

• MAD homolog 9
• MAD, mothers against decapentaplegic homolog 9 (Drosophila)
• Madh6
• MADH6mothers against decapentaplegic homolog 9
• MADH9
• MADH9Mothers against decapentaplegic, drosophila, homolog of, 9
• Mothers against DPP homolog 9
• SMAD 9
• SMAD family member 9SMAD8B
• SMAD, mothers against DPP homolog 9 (Drosophila)
• SMAD, mothers against DPP homolog 9
• SMAD8
• SMAD8A
• Smad9

Background

SMAD9 (SMA protein and Mothers Against Decapentaplegic 9; also SMAD8 and MAHD6) is a 55-60 kDa R-Group member of the dwarfin/SMAD family of proteins. It is expressed by BMP-responsive cells, and serves to transmit information from the BMP receptor (BMPR) to the nucleus. In particular, following BMPR activation, SMAD9 is phosphorylated by the BMPR on its C-terminus, promoting heterodimerization with SMAD4 and its translocation into the nucleus. Here, SMAD9 binds to DNA and participates in gene activation. Human SMAD9 is 467 amino acids (aa) in length. It contains a DNA-binding domain termed MH1 (aa 16-140) plus a receptor/transcription factor binding domain termed MH2 (aa 273-467). The C-terminus contains two phosphorylation sites at Ser465 and Ser467. There is one splice form that shows a deletion of aa 224-260. Over aa 158-223, human and mouse SMAD9 share 82% identy in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.