Human Smad3 ELISA Kit (Colorimetric)

ELISA: Human Smad3 ELISA Kit (Colorimetric) [NBP2-76622] - Samples were spiked with high concentrations of Human Smad3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Smad3 ELISA Kit (Colorimetric) [NBP2-76622] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.625 - 40ng/mL
• Sensitivity: 0.375ng/mL

Human Smad3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Smad3/MADH3 in samples. No significant cross-reactivity or interference between Human Smad3/MADH3 and analogues was observed.
• Standard Curve Range: 0.625 - 40ng/mL
• Sensitivity: 0.375ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.1
• Intra-Assay: CV% < 5.62
• Spike Recovery: 86-107%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SMAD3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Smad3 ELISA Kit (Colorimetric)

• DKFZp586N0721
• DKFZp686J10186
• hMAD-3
• hSMAD3
• HsT17436
• JV15-2MGC60396
• MAD homolog 3
• mad protein homolog
• MAD, mothers against decapentaplegic homolog 3 (Drosophila)
• MAD, mothers against decapentaplegic homolog 3
• mad3
• MADH3mad homolog JV15-2
• mothers against decapentaplegic homolog 3
• Mothers against DPP homolog 3
• SMA- and MAD-related protein 3
• SMAD 3
• SMAD family member 3HSPC193
• SMAD, mothers against DPP homolog 3 (Drosophila)
• SMAD, mothers against DPP homolog 3
• Smad3

Background

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Smad3 ELISA Kit (NBP2-76622)(1)

Publication using NBP2-76622

• Michele Vacca, Jack Leslie, Samuel Virtue, Brian Y H Lam, Olivier Govaere, Dina Tiniakos, Sophie Snow, Susan Davies, Kasparas Petkevicius, Zhen Tong, Vivian Peirce, Mette Juul Nielsen, Zsuzsanna Ament, Wei Li, Tomasz Kostrzewski, Diana Julie Leeming, Vlad Ratziu, Michael E D Allison, Quentin M Anstee, Julian L Griffin, Fiona Oakley, Antonio Vidal-Puig Bone morphogenetic protein 8B promotes the progression of non-alcoholic steatohepatitis. Nature metabolism 2020-12-31 [PMID: 32694734]

FAQs for Smad3 ELISA Kit (NBP2-76622). (Showing 1 - 2 of 2 FAQ).

1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
   • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SMAD3