Rat Smad3 ELISA Kit (Colorimetric)

ELISA: Rat Smad3 ELISA Kit (Colorimetric) [NBP2-76621] - Samples were spiked with high concentrations of Rat Smad3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat Smad3 ELISA Kit (Colorimetric) [NBP2-76621] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.156 - 10ng/mL
• Sensitivity: 0.094ng/mL

Rat Smad3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat Smad3/MADH3 in samples. No significant cross-reactivity or interference between Rat Smad3/MADH3 and analogues was observed.
• Standard Curve Range: 0.156 - 10ng/mL
• Sensitivity: 0.094ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.6
• Intra-Assay: CV% < 5.22
• Spike Recovery: 85-102%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SMAD3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat Smad3 ELISA Kit (Colorimetric)

• DKFZp586N0721
• DKFZp686J10186
• hMAD-3
• hSMAD3
• HsT17436
• JV15-2MGC60396
• MAD homolog 3
• mad protein homolog
• MAD, mothers against decapentaplegic homolog 3 (Drosophila)
• MAD, mothers against decapentaplegic homolog 3
• mad3
• MADH3mad homolog JV15-2
• mothers against decapentaplegic homolog 3
• Mothers against DPP homolog 3
• SMA- and MAD-related protein 3
• SMAD 3
• SMAD family member 3HSPC193
• SMAD, mothers against DPP homolog 3 (Drosophila)
• SMAD, mothers against DPP homolog 3
• Smad3

Background

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Smad3 ELISA Kit (NBP2-76621)(1)

Publication using NBP2-76621

• El-Waseif E, Sharawy M, Suddek G The modulatory effect of sodium molybdate against cisplatin-induced CKD: Role of TGF-beta/Smad signaling pathway Life Sciences 2022-07-01 [PMID: 35917941] (ELISA)

FAQs for Smad3 ELISA Kit (NBP2-76621). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
   • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Bioinformatics

• Gene Symbol: SMAD3