Smad3 Antibody (SY25-01)

Western Blot: Smad3 Antibody (SY25-01) [NBP2-67434] - Western blot analysis of Smad3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab

Immunocytochemistry/Immunofluorescence: Smad3 Antibody (SY25-01) [NBP2-67434] - Staining Smad3 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Smad3 Antibody (SY25-01) [NBP2-67434] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Smad3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Smad3 Antibody (SY25-01) [NBP2-67434] - Analysis of Hela cells with Smad3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Smad3 Antibody (SY25-01) [NBP2-67434] - Analysis of Smad3 on A549 cell lysates using anti-Smad3 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Smad3 Antibody (SY25-01) [NBP2-67434] - Staining Smad3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Smad3 Antibody (SY25-01) [NBP2-67434] - Staining Smad3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Smad3 Antibody (SY25-01) [NBP2-67434] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Smad3 Antibody (SY25-01) [NBP2-67434] - Analysis of paraffin-embedded human lung cancer tissue using anti-Smad3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Smad3 Antibody (SY25-01) [NBP2-67434] - Analysis of paraffin-embedded mouse brain tissue using anti-Smad3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Smad3 Antibody (SY25-01) [NBP2-67434] - Analysis of paraffin-embedded mouse liver tissue using anti-Smad3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Smad3 Antibody (SY25-01) [NBP2-67434] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Smad3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Smad3 Antibody (SY25-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Smad3 aa 181-230 / 425. (SwissProt: P84022 Human; SwissProt: Q8BUN5 Mouse; SwissProt: P84025 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMAD3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Smad3 Antibody (SY25-01)

• DKFZp586N0721
• DKFZp686J10186
• hMAD-3
• hSMAD3
• HsT17436
• JV15-2MGC60396
• MAD homolog 3
• mad protein homolog
• MAD, mothers against decapentaplegic homolog 3 (Drosophila)
• MAD, mothers against decapentaplegic homolog 3
• Mad3
• MADH3mad homolog JV15-2
• mothers against decapentaplegic homolog 3
• Mothers against DPP homolog 3
• SMA- and MAD-related protein 3
• SMAD 3
• SMAD family member 3HSPC193
• SMAD, mothers against DPP homolog 3 (Drosophila)
• SMAD, mothers against DPP homolog 3
• Smad3

Background

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Smad3 Antibody (NBP2-67434). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
   • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Bioinformatics

• Gene Symbol: SMAD3