Smad3 Antibody (378611) [Unconjugated]

Smad3 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using 15 µg/mL Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PC‑3 human prostate cancer cell line was stained with Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Smad3 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rat Anti-Human Smad3 Monoclonal Antibody (Catalog # MAB4038) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: IHC, CyTOF-ready, Flow, ICC/IF
• Clone: 378611
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Smad3 Antibody (378611) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Smad3
  Ser2-Ala230
  Accession # P84022
• Specificity: Detects human Smad3 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: SMAD3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad3 Antibody (378611) [Unconjugated]

• DKFZp586N0721
• DKFZp686J10186
• hMAD-3
• hSMAD3
• HsT17436
• JV15-2MGC60396
• MAD homolog 3
• mad protein homolog
• MAD, mothers against decapentaplegic homolog 3 (Drosophila)
• MAD, mothers against decapentaplegic homolog 3
• mad3
• MADH3mad homolog JV15-2
• mothers against decapentaplegic homolog 3
• Mothers against DPP homolog 3
• SMA- and MAD-related protein 3
• SMAD 3
• SMAD family member 3HSPC193
• SMAD, mothers against DPP homolog 3 (Drosophila)
• SMAD, mothers against DPP homolog 3
• Smad3

Background

Smad3 is phosphorylated in cells stimulated with TGF-beta , complexes with Smad4, and translocates to the nucleus to upregulate gene transcription. Smad3 is critical for signaling fibrosis and wound healing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad3 Antibody (MAB4038)(3)

Publications using MAB4038

• S Berto, MR Fontenot, S Seger, F Ayhan, E Caglayan, A Kulkarni, C Douglas, CA Tamminga, BC Lega, G Konopka Gene-expression correlates of the oscillatory signatures supporting human episodic memory encoding Nature Neuroscience, 2021-03-08;0(0):. 2021-03-08 [PMID: 33686299] (Flow Cytometry, IHC, Human)
• P Vijayaraj, A Minasyan, A Durra, S Karumbayar, M Mehrabi, CJ Aros, SD Ahadome, DW Shia, K Chung, JM Sandlin, KF Darmawan, KV Bhatt, CC Manze, MK Paul, DC Wilkinson, W Yan, AT Clark, TM Rickabaugh, WD Wallace, TG Graeber, R Damoiseaux, BN Gomperts Modeling Progressive Fibrosis with Pluripotent Stem Cells Identifies an Anti-fibrotic Small Molecule Cell Rep, 2019-12-10;29(11):3488-3505.e9. 2019-12-10 [PMID: 31825831] (ICC, Western Blot, Human)
• Yeh YH, Kuo CT, Chan TH, Chang GJ, Qi XY, Tsai F, Nattel S, Chen WJ Transforming growth factor-beta and oxidative stress mediate tachycardia-induced cellular remodelling in cultured atrial-derived myocytes. Cardiovasc. Res., 2011-02-02;91(1):62-70. 2011-02-02 [PMID: 21289011] (Western Blot, Mouse)

FAQs for Smad3 Antibody (MAB4038). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
   • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Bioinformatics

• Gene Symbol: SMAD3
• Uniprot:
  • P84022()