Smad3 Antibody (1B7)

Western Blot: Smad3 Antibody (1B7) [H00004088-M07] - Analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M07), clone 1B7. Lane 1: SMAD3 transfected lysatE (48 KDa). Lane 2: Non-transfected lysate.

Western Blot: Smad3 Antibody (1B7) [H00004088-M07] - Analysis of SMAD3 expression in HeLa (Cat # L013V1).

Western Blot: Smad3 Antibody (1B7) [H00004088-M07] - Analysis of SMAD3 expression in PC-12 (Cat # L012V1).

Western Blot: Smad3 Antibody (1B7) [H00004088-M07] - Analysis of SMAD3 expression in Raw 264.7 (Cat # L024V1).

Western Blot: Smad3 Antibody (1B7) [H00004088-M07] - Analysis of SMAD3 expression in NIH/3T3 (Cat # L018V1).

ELISA: Smad3 Antibody (1B7) [H00004088-M07] - Detection limit for recombinant GST tagged SMAD3 is approximately 3ng/ml as a capture antibody.

Product Discontinued

Smad3 Antibody (1B7) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SMAD3 (NP_005893, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCE
• Specificity: SMAD3 - SMAD, mothers against DPP homolog 3 (Drosophila)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMAD3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate for WB. It has been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Smad3 Antibody (1B7)

• DKFZp586N0721
• DKFZp686J10186
• hMAD-3
• hSMAD3
• HsT17436
• JV15-2MGC60396
• MAD homolog 3
• mad protein homolog
• MAD, mothers against decapentaplegic homolog 3 (Drosophila)
• MAD, mothers against decapentaplegic homolog 3
• mad3
• MADH3mad homolog JV15-2
• mothers against decapentaplegic homolog 3
• Mothers against DPP homolog 3
• SMA- and MAD-related protein 3
• SMAD 3
• SMAD family member 3HSPC193
• SMAD, mothers against DPP homolog 3 (Drosophila)
• SMAD, mothers against DPP homolog 3
• Smad3

Background

The protein encoded by the Smad3 gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Smad3 Antibody (H00004088-M07). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
   • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Bioinformatics

• Gene Symbol: SMAD3
• Entrez:
  • 4088(Human)
• OMIM:
  • 603109(Human)
• Uniprot:
  • NP_005893(Human)