Smad2 [p Ser467] Antibody - BSA Free

Western Blot: Smad2 [p Ser467] Antibody [NBP2-54771] - Analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Ser467).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Smad2 [p Ser467] Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Smad2 [p Ser467] Antibody - BSA Free (NBP2-54771) is a polyclonal antibody validated for use in IHC, WB and Simple Western. Anti-Smad2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Smad2 (Phospho-Ser467) antibody was raised against a peptide sequence around phosphorylation site of serine 467 (C-S-S-M-S (p) ) derived from Human Smad2.
• Modification: p Ser467
• Specificity: This antibody detects endogenous level of Smad2 only when phosphorylated at serine 467.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD2
• Purity: Epitope affinity purified

Applications/Dilutions

• Dilutions:
  • Simple Western
  • Western Blot 1:500-1:1000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Bladder, separated by Size
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.
• Buffer: PBS (pH 7.4) (without Mg2+ and Ca2+), 150mM NaCl, 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Epitope affinity purified

Alternate Names for Smad2 [p Ser467] Antibody - BSA Free

• hMAD-2
• hSMAD2
• JV18
• JV18-1
• JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
• MAD homolog 2
• Mad protein homolog
• Mad, mothers against decapentaplegic homolog 2
• MADH2
• MADH2MGC34440
• MADR2
• MADR2MGC22139
• Mad-related protein 2
• mother against DPP homolog 2
• mothers against decapentaplegic homolog 2
• Mothers against DPP homolog 2
• pSMAD
• Sma- and Mad-related protein 2
• SMAD 2
• SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
• SMAD, mothers against DPP homolog 2
• Smad2
• Smox

Background

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad2 Antibody (NBP2-54771)(2)

Publications using NBP2-54771

• Salemi S, Schori L, Gerwinn T et al. Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction International Journal of Molecular Sciences 2023-02-24 [PMID: 36901894] (IHC-P, Human)
• Mukhi D, Kolligundla LP, Maruvada S et al. Growth hormone induces transforming growth factor-beta 1 in podocytes: Implications in podocytopathy and proteinuria Biochimica et biophysica acta. Molecular cell research 2022-11-15 [PMID: 36400249] (WB, Human)

Bioinformatics

• Gene Symbol: SMAD2
• Entrez:
  • NP_001003652.1(Human)