Smad2 Antibody

Images

 
Western Blot: SMAD2 Antibody [NB100-56462] - analysis using SMAD2 antibody. Lysate from rat muscle in the 1) absence and 2) presence of immunizing peptide probed with SMAD2 antibody at 3 ug/ml.
Immunohistochemistry-Paraffin: SMAD2 Antibody [NB100-56462] - analysis using SMAD2 antibody. Formalin-fixed, paraffin-embedded mouse testis tissue probed with SMAD2 antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Smad2 Antibody Summary

Immunogen
Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 protein was used as the immunogen.
Specificity
The SMAD2 amino acid sequence used as immunogen is 100% homologous in human (isoform 1: 467 amino acids; isoform 2: 437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Positive Control
Smad2 Lysate (NBL1-16220)
Publications
Read Publication using
NB100-56462 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Reacts with Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for Smad2 Antibody

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2
  • MADH2MGC34440
  • MADR2
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smox

Background

SMAD2 is an intracellular protein belonging to the Dwarfin (DWA/B)/SMAD family. SMAD2 associates with SMAD4 for translocation to nucleus. It acts as an intracellular mediator of TGFbeta family of cytokines and activin type 1 receptor (ACVR1C). Hence it regulates multiple cellular processes like cell growth, proliferation, differentiation, and apoptosis and also cooperates with transcription factors to regulate expression of defined genes. Apart from playing an important role in TGFbeta1-induced CTGF expression they also act as markers of EMT in human PTECs. It also acts as an integrator of multiple signals in the regulation of NOS3 expression. It is ubiquitously expressed in most of the tissues and is responsible for colorectal, hepatocellular, lung and breast carcinomas as well as cervical cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad2 Antibody (NB100-56462)(1)

We have publications tested in 1 confirmed species: Chicken.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(1)
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(1)
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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMAD2
Entrez
Uniprot