Smad2 Overexpression Lysate (Native)

Images

 
Western Blot: SMAD2 Overexpression Lysate (Adult Normal) [NBL1-16220] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMAD2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Smad2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206526 and based on accession number NM_001003652. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens SMAD family member 2 (SMAD2), transcript variant 2, mRNA.
Gene
SMAD2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Smad2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Smad2 Overexpression Lysate (Native)

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2
  • MADH2MGC34440
  • MADR2
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smox

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-77836
Species: Hu, Mu, Rt, Po, Bv, Ch, Xp, Ze
Applications: WB, ELISA, Flow, IHC, IHC-P
AF2097
Species: Hu
Applications: WB, ChIP, ICC
MAB2029
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
AF3025
Species: Hu
Applications: WB, ICC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-56656
Species: Hu, Mu, Rt
Applications: WB
NBP2-45808
Species: Hu
Applications: IHC
NB100-724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-19773
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC

Publications for Smad2 Lysate (NBL1-16220) (0)

There are no publications for Smad2 Lysate (NBL1-16220).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Smad2 Lysate (NBL1-16220) (0)

There are no reviews for Smad2 Lysate (NBL1-16220). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Smad2 Lysate (NBL1-16220) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Smad2 Products

Bioinformatics Tool for Smad2 Lysate (NBL1-16220)

Discover related pathways, diseases and genes to Smad2 Lysate (NBL1-16220). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Smad2 Lysate (NBL1-16220)

Discover more about diseases related to Smad2 Lysate (NBL1-16220).
 

Pathways for Smad2 Lysate (NBL1-16220)

View related products by pathway.

PTMs for Smad2 Lysate (NBL1-16220)

Learn more about PTMs related to Smad2 Lysate (NBL1-16220).
 

Research Areas for Smad2 Lysate (NBL1-16220)

Find related products by research area.

Blogs on Smad2

There are no specific blogs for Smad2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Smad2 Antibody
NB100-56462

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Smad2 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SMAD2
Entrez