SMAD1/9 Antibody (SD2090)

Western Blot: SMAD1/9 Antibody (SD2090) [NBP2-67768] - Analysis of Smad1 on different lysates using anti-Smad1 antibody at 1/1,000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Hela

Immunocytochemistry/Immunofluorescence: SMAD1/9 Antibody (SD2090) [NBP2-67768] - Staining Smad1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMAD1/9 Antibody (SD2090) [NBP2-67768] - Analysis of paraffin-embedded human stomach tissue using anti-SMAD1/9 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/Immunofluorescence: SMAD1/9 Antibody (SD2090) [NBP2-67768] - Staining Smad1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SMAD1/9 Antibody (SD2090) [NBP2-67768] - Staining Smad1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SMAD1/9 Antibody (SD2090) [NBP2-67768] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad1 antibody. Counter stained with hematoxylin.

Product Discontinued

SMAD1/9 Antibody (SD2090) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human SMAD1/9 aa 221-270. (SwissProt: Q15797 Human)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMAD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SMAD1/9 Antibody (SD2090)

• BSP1
• BSP-1
• hSMAD1
• JV41
• JV4-1
• JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
• MAD homolog 1
• MADH1
• MADH1JV41
• MADR1
• MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
• Mad-related protein 1
• mothers against decapentaplegic homolog 1
• Mothers against DPP homolog 1
• SMAD family member 1
• SMAD family member 1SMAD 1
• SMAD, mothers against DPP homolog 1
• Smad1
• TGF-beta signaling protein 1
• transforming growth factor-beta signaling protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SMAD1/9 Antibody (NBP2-67768) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/16/2022

Summary

• Application: Western Blot
• Sample Tested: Skeletal muscle
• Species: Human
• Lot: SD2090

Comments

• Comments: 1/1000 antibody dilution

Bioinformatics

• Gene Symbol: SMAD1