Slit2 Antibody (SR2034) - BSA Free

Western Blot: Slit2 Antibody (SR2034) [NBP3-21803] - Western blot analysis using NBP3-21803 in SH-SY5Y cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clone: SR2034
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Slit2 Antibody (SR2034) - BSA Free Summary

• Description: Novus Biologicals Rabbit Slit2 Antibody (SR2034) - BSA Free (NBP3-21803) is a recombinant monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Slit2 (Uniprot #: O94813)
• Specificity: Detects endogenous levels of total Slit2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SLIT2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Slit2 Antibody (SR2034) - BSA Free

• FLJ14420
• SLIL3
• slit (Drosophila) homolog 2
• slit homolog 2 (Drosophila)
• slit homolog 2 protein
• Slit2
• Slit-2SLIL3

Background

Slit proteins are ligands at the Roundabout (Robo) receptors and act as guidance cues in axonal migration/navigation during neural development, at the ventral midline of the neural tube. Slit1 and Slit2 are essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. A number of cleavage products are reported in the literature for Slit2 protiens (following alternate splicing). The C-terminal cleavage proteins are more diffusible than the larger N-terminal protein that is more tightly cell associated. Slit2 protein is expressed in fetal lung and kidney, and adult spinal cord. Weak expression in adult adrenal gland, thyroid, trachea.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLIT2