SLFN5 Antibody

Images

 
Orthogonal Strategies: Western Blot: SLFN5 Antibody [NBP1-81178] - Analysis in human cell lines SK-MEL-30 and Caco-2. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.
Immunocytochemistry/ Immunofluorescence: SLFN5 Antibody [NBP1-81178] - Staining of human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human skeletal muscle shows weak nuclear positivity in myocytes as expected.
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: SLFN5 Antibody [NBP1-81178] - Staining of human endometrium shows strong nuclear positivity in glandular cells.A
Simple Western: SLFN5 Antibody [NBP1-81178] - Simple Western lane view shows a specific band for SLFN5 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions ...read more
Simple Western: SLFN5 Antibody [NBP1-81178] - Electropherogram image(s) of corresponding Simple Western lane view. SLFN5 antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

SLFN5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ERHGVGLDVPPIFRSHLDKMQKENHFLIFVKSWNTEAGVPLATLCSNLYHRERTSTDVMDSQEALAFLKCRTQTPTNINVSNSLGPQAAQGSVQYEGNINVSAAALFDRKRLQYLEKLNLPESTHVEFVMFSTDVSHCVKD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLFN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Simple Western 1:20
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SLFN5 Protein (NBP1-81178PEP)
Publications
Read Publications using
NBP1-81178 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLFN5 Antibody

  • MGC150611
  • MGC150612
  • MGC19764
  • schlafen family member 5

Background

SLFN5 may have a role in hematopoeitic cell differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLFN5 Antibody (NBP1-81178)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SLFN5 Antibody (NBP1-81178) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLFN5
Entrez