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CCDC8 Antibody

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Immunocytochemistry/Immunofluorescence: CCDC8 Antibody [NBP1-85085] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunocytochemistry/ Immunofluorescence: CCDC8 Antibody [NBP1-85085] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: CCDC8 Antibody [NBP1-85085] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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CCDC8 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAPAVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVSAAQG
Specificity
Specificity of human CCDC8 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCDC8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25 - 2 ug/mL
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. This CCDC8 Antibody is validated for IHC-Fr from a verified customer review.
Control Peptide
CCDC8 Protein (NBP1-85085PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-85085 in the following applications:
Publications
Read Publication using NBP1-85085.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CCDC8 Antibody

  • coiled-coil domain containing 8
  • coiled-coil domain-containing protein 8
  • DKFZp564K0322

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu
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Publications for CCDC8 Antibody (NBP1-85085)(1)


Review for CCDC8 Antibody (NBP1-85085) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-85085:
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Immunohistochemistry-Frozen CCDC8 NBP1-85085
Enlarge 		4
reviewed by:
Valeria Di Leo
 IHC-Fr Human 11/05/2020
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSkeletal muscle
SpeciesHuman
LotNBP1-85085

Comments

CommentsFixative: 4% paraformaldehyde
Permeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins)
Protein blocking: 10% NGS diluted in TBST
Endogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc)
Dilution: 1/50
Incubation time: 19 hour(s) and 0 minute(s) · Diluent TBST – NGS 10%
Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, anti-Rabbit isotype IgG, Host species: Goat, Clonality: Polyclonal

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FAQs for CCDC8 Antibody (NBP1-85085) (0)

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Immunohistochemistry-Frozen CCDC8 NBP1-85085
Enlarge
 Valeria Di Leo
11/05/2020
Application: IHC-Fr
Species: Human
read this review

Bioinformatics

Gene Symbol CCDC8