Publications for CCDC8 Antibody (NBP1-85085)(1)
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CCDC8 Antibody (NBP1-85085) (1)
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Valeria Di Leo IHC-Fr
Application Immunohistochemistry-Frozen Sample Tested Skeletal muscle Species Human Lot NBP1-85085 Comments
Comments Fixative: 4% paraformaldehyde Permeabilization: Methanol gradient (70% for 10 mins, 95% for 10 mins, 100% for 20 mins, 95% for 10 mins, 70% for 10 mins) Protein blocking: 10% NGS diluted in TBST Endogenous biotin blocking: Avidin D solution for 15 mins, Biotin for 15 mins (Vector Laboratories, Inc) Dilution: 1/50 Incubation time: 19 hour(s) and 0 minute(s) · Diluent TBST – NGS 10% Secondary antibody: Life tech/ThermoFisher, A11006, Alexa 488, anti-Rabbit isotype IgG, Host species: Goat, Clonality: Polyclonal
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