![Immunocytochemistry/Immunofluorescence: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SLC45A3-Prostein-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89630-img0007.jpg "Immunocytochemistry/Immunofluorescence: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit SLC45A3/Prostein Antibody - BSA Free (NBP1-89630) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-SLC45A3/Prostein Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: AEEAALGPTEPAEGLSAPSLSPHCCPCRARLAFRNLGALLPRLHQLCCRMP |
| Marker | Prostate Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC45A3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
| Publication using NBP1-89630 | Applications | Species |
|---|---|---|
| Cai C, Wang W, Tu Z. Aberrantly DNA Methylated-Differentially Expressed Genes and Pathways in Hepatocellular Carcinoma. J. Cancer 2019-01-08 [PMID: 30719129] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SLC45A3 |
![Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human liver, placenta, prostate and testis using Anti-SLC45A3 antibody NBP1-89630 (A) shows similar protein distribution across tissues to independent antibody NBP1-89629 (B).](http://images.novusbio.com/fullsize/SLC45A3-Prostein-Antibody-Immunohistochemistry-Paraffin-NBP1-89630-img0015.jpg "Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human liver, placenta, prostate and testis using Anti-SLC45A3 antibody NBP1-89630 (A) shows similar protein distribution across tissues to independent antibody NBP1-89629 (B).")
![Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SLC45A3-Prostein-Antibody-Immunohistochemistry-Paraffin-NBP1-89630-img0017.jpg "Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/SLC45A3-Prostein-Antibody-Immunohistochemistry-Paraffin-NBP1-89630-img0014.jpg "Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/SLC45A3-Prostein-Antibody-Immunohistochemistry-Paraffin-NBP1-89630-img0016.jpg "Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human placenta shows no positivity in trophoblastic cells as expected.")
![Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/SLC45A3-Prostein-Antibody-Immunohistochemistry-Paraffin-NBP1-89630-img0018.jpg "Immunohistochemistry-Paraffin: SLC45A3/Prostein Antibody [NBP1-89630] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")