SLC39A8/ZIP8 Antibody - BSA Free

Western Blot: SLC39A8/ZIP8 Antibody [NBP2-93479] - Analysis of extracts of various cell lines, using SLC39A8/ZIP8 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: SLC39A8/ZIP8 Antibody - BSA Free [NBP2-93479] - Immunofluorescence analysis of L929 cells using SLC39A8/ZIP8 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLC39A8/ZIP8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SLC39A8/ZIP8 Antibody - BSA Free (NBP2-93479) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-300 of human ZIP8 (NP_071437.3). QLIPEAFGFDPKVDSYVEKAVAVFGGFYLLFFFERMLKMLLKTYGQNGHTHFGNDNFGPQEKTHQPKALPAINGVTCYANPAVTEANGHIHFDNVSVVSLQDGKKEPSSCTCLKGPKLSEI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC39A8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SLC39A8/ZIP8 Antibody - BSA Free

• BCG induced integral membrane protein BIGM103
• BIGM103BCG-induced integral membrane protein in monocyte clone 103 protein
• LIV-1 subfamily of ZIP zinc transporter 6
• LZT-Hs6
• solute carrier family 39 (metal ion transporter), member 8
• solute carrier family 39 (zinc transporter), member 8
• Solute carrier family 39 member 8
• zinc transporter ZIP8
• ZIP8
• ZIP-8
• Zrt- and Irt-like protein 8

Background

The SLC39A8 gene encodes a member of the SLC39 family of solute-carrier genes, which show structural characteristics of zinc transporters. The encoded protein is glycosylated and found in the plasma membrane and mitochondria, and functions in the cellular import

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC39A8