SLC39A7/ZIP7 Antibody - BSA Free

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: SLC39A7/ZIP7 Antibody [NBP1-76504] - Immunofluorescence of ZIP7 in human brain tissue with ZIP7 antibody at 20 ug/mL.

Immunohistochemistry: SLC39A7/ZIP7 Antibody [NBP1-76504] - Immunohistochemistry of ZIP7 in human brain tissue with ZIP7 antibody at 2.5 ug/mL.

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), ZIP7 from Company B (4 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: SLC39A7/ZIP7 Antibody [NBP1-76504] - Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: ZIP7, NBP1-76504 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SLC39A7/ZIP7 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human SLC39A7/ZIP7. The immunogen is located within amino acids 20-70 of SLC39A7/ZIP7. Amino Acid Sequence: HDDLQEDFHGHSHRHSH
• Specificity: Human SLC39A7/ZIP7 has one isoform (469aa, 50kD). Mouse SLC39A7/ZIP7 has one isoform (476aa, 51kD) and Rat SLC39A7/ZIP7 also has one isoform (468aa, 50kD). NBP1-76504 can detect human, mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC39A7
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/mL
• Application Notes: Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Predicted: 50kD; Observed: 50kD
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  SLC39A7/ZIP7 Peptide (NBP1-76504PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SLC39A7/ZIP7 Antibody - BSA Free

• D6S115E
• D6S2244EReally interesting new gene 5 protein
• H2-KE4
• HKE4Solute carrier family 39 member 7
• HLA class II region expressed gene KE4
• Ke4 gene, mouse, human homolog of
• KE4
• RING5Histidine-rich membrane protein Ke4
• solute carrier family 39 (zinc transporter), member 7
• zinc transporter SLC39A7
• ZIP7

Background

The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC39A7
• Uniprot:
  • NP_001070984(Human)