SLC39A14 Antibody - BSA Free

Western Blot: SLC39A14 Antibody [NBP2-93483] - Western blot analysis of extracts of various cell lines, using SLC39A14 antibody (NBP2-93483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry: SLC39A14 Antibody - Azide and BSA Free [NBP2-93483] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using SLC39A14 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: SLC39A14 Antibody - Azide and BSA Free [NBP2-93483] - Immunohistochemistry analysis of paraffin-embedded Rat liver using SLC39A14 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLC39A14 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SLC39A14 Antibody - BSA Free (NBP2-93483) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 101-200 of human SLC39A14 (NP_001121903.1). EENEQTEEGRPSAVE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC39A14
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 -1:200
  • Simple Western
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SLC39A14 Antibody - BSA Free

• Irt-like protein 14
• LIV-1 subfamily of ZIP zinc transporter 4
• LZT-Hs4
• solute carrier family 39 (metal ion transporter), member 14
• solute carrier family 39 (zinc transporter), member 14
• solute carrier family 39 member 14
• zinc transporter ZIP14
• ZIP14
• ZIP-14
• zrt- and Irt-like protein 14

Background

Zinc is an essential cofactor for hundreds of enzymes. It is involved in protein, nucleic acid, carbohydrate, and lipid metabolism, as well as in the control of gene transcription, growth, development, and differentiation. SLC39A14 belongs to a subfamily of proteins that show structural characteristics of zinc transporters (Taylor and Nicholson, 2003 (PubMed 12659941)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC39A14