SLC20A1 Antibody

Images

 
Western Blot: SLC20A1 Antibody [NBP1-32252] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE SLC20A1 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: SLC20A1 Antibody [NBP1-32252] - Paraffin-embedded mouse mammary gland. SLC20A1 antibody [N3C2], Internal diluted at 1:500.
Western Blot: SLC20A1 Antibody [NBP1-32252] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: SLC20A1 Antibody [NBP1-32252] - Paraffin-embedded AGS xenograft. SLC20A1 antibody [N3C2], Internal dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Fe, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.48 mg/ml

Order Details

SLC20A1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SLC20A1. The exact sequence is proprietary.
Localization
Membrane, Multi-pass membrane protein
Predicted Species
Rat (94%), Bovine (95%), Feline (95%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC20A1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (95%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.48 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SLC20A1 Antibody

  • FLJ41426
  • Gibbon ape leukemia virus receptor 1
  • Glvr-1
  • GLVR1DKFZp686J2397
  • Leukemia virus receptor 1 homolog
  • Phosphate transporter 1
  • PiT-1PIT1
  • sodium-dependent phosphate transporter 1
  • solute carrier family 20 (phosphate transporter), member 1
  • Solute carrier family 20 member 1

Background

Retrovirus receptors allow infection of human and murine cells by various retroviruses. The receptors that have been identified at the molecular level include CD4 (MIM 186940) for human immunodeficiency virus, Rec1 for murine ecotropic virus, and GLVR1 for gibbon ape leukemia virus (see MIM 182090). These 3 proteins show no homology to one another at the DNA or protein level. GLVR1 is a sodium-dependent phosphate symporter.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

FAQs for SLC20A1 Antibody (NBP1-32252). (Showing 1 - 1 of 1 FAQ).

  1. The predicted molecular weight of SLC20A1 protein is about 74 kDa. Why can this antibody (NBP1-32252) detect two bands in different cell types? The translational modification seems not to explain these variations in MW.
    • SLC20A1 is a transmembrane protein and, as well as post-translational modification (1 extra phosphorylation site), another factor responsible for its differential migration in different species might be the differences in the integration of this protein in the membrane. It has 10 transmembrane regions and 11 topological regions in the sequence and the position of amino acids varies in mouse and human with respect to these regions in this protein.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SLC20A1
Entrez
Uniprot