SLBP Antibody - BSA Free

Western Blot: SLBP Antibody [NBP3-12936] - HepG2 whole cell and cytoplasm extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SLBP antibody (NBP3-12936) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: SLBP Antibody [NBP3-12936] - SLBP antibody detects SLBP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLBP protein stained by SLBP antibody (NBP3-12936) diluted at 1:500. Blue: Hoechst 33342 staining.

Knockdown Validated: SLBP Antibody [NBP3-12936] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SLBP antibody (NBP3-12936) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SLBP antibody (NBP3-12936) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm
• Applications: WB, ICC/IF, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SLBP Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SLBP. The exact sequence is proprietary.
• Predicted Species: Mouse (95%), Rat (93%), Porcine (90%), Chimpanzee (100%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLBP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Knockdown Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for SLBP Antibody - BSA Free

• histone binding protein
• histone RNA hairpin-binding protein
• histone stem-loop binding protein
• Histone stem-loop-binding protein
• histone
• stem-loop binding protein

Background

SLBP encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLBP