Sphingosine Kinase 1/SPHK1 Antibody (1D6)

Images

 
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6. Analysis of SPHK1 expression in K-562.
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6 Western Blot analysis of SPHK1 expression in A-431 ( Cat # L015V1 ).
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - Analysis of SPHK1 expression in transfected 293T cell line by SPHK1 monoclonal antibody (M01), clone 1D6.Lane 1: SPHK1 transfected lysate(43.9 ...read more
ELISA: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - Detection limit for recombinant GST tagged SPHK1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Sphingosine Kinase 1/SPHK1 Antibody (1D6) Summary

Immunogen
SPHK1 (AAH08040, 1 a.a. - 384 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
Specificity
SPHK1 (1D6)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SPHK1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Publications
Read Publications using H00008877-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Sphingosine Kinase 1/SPHK1 Antibody (1D6)

  • EC 2.7.1.91
  • SK1
  • sphingosine kinase 1
  • SPHK1
  • SPHKSK 1
  • SPK 1
  • SPK
  • SPK1

Background

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sphingosine Kinase 1/SPHK1 Antibody (H00008877-M01)(11)

We have publications tested in 1 application: WB.


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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using H00008877-M01 Applications Species
Zhang H, Wang Q, Zhao Q, Di W. MiR-124 inhibits the migration and invasion of ovarian cancer cells by targeting SphK1. J Ovarian Res. 2013 [PMID: 24279510] (WB) WB
Bohm A, Flosser A, Ermler S et al. Factor-Xa-induced mitogenesis and migration require sphingosine kinase activity and S1P formation in human vascular smooth muscle cells. Cardiovasc Res. 2013 May 07 [PMID: 23658376]
Sheu JJ, Lee CC, Hua CH et al. LRIG1 modulates aggressiveness of head and neck cancers by regulating EGFR-MAPK-SPHK1 signaling and extracellular matrix remodeling. Oncogene. 2013 Apr 29 [PMID: 23624915]
Schroder M, Richter C, Juan MH et al. The sphingosine kinase 1 and S1P1 axis specifically counteracts LPS-induced IL-12p70 production in immune cells of the spleen. Mol Immunol. 2011 Mar 22 [PMID: 21435724]
Asher V, Warren A, Shaw R et al. The role of Eag and HERG channels in cell proliferation and apoptotic cell death in SK-OV-3 ovarian cancer cell line. Cancer Cell Int. 2011 Mar 10 [PMID: 21392380]
Ali-Rahmani F, Hengst JA, Connor JR, Schengrund CL. Effect of HFE Variants on Sphingolipid Expression by SH-SY5Y Human Neuroblastoma Cells. Neurochem Res. 2011 Jan 18 [PMID: 21243428]
Schenten V, Melchior C, Steinckwich N et al. Sphingosine kinases regulate NOX2 activity via p38 MAPK-dependent translocation of S100A8/A9. J Leukoc Biol. 2011 Jan 13 [PMID: 21233411]
Sinha UK, Schorn VJ, Hochstim C et al. Increased radiation sensitivity of head and neck squamous cell carcinoma with sphingosine kinase 1 inhibition. Wiley InterScience. 2011 Feb [PMID: 20848438]
Wang S, Zhang Z, Lin X et al. A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart. Glycobiology 20(4):473-84. 2010 Jan. [PMID: 20008963]
Ter Braak M, Danneberg K, Lichte K et al. G{alpha}q-mediated plasma membrane translocation of sphingosine kinase-1 and cross-activation of S1P receptors. Biochim Biophys Acta. 2009 Feb 03 [PMID: 19416644]
Show All 11 Publications.

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Bioinformatics

Gene Symbol SPHK1
Entrez
OMIM
Uniprot