SKA2 Antibody - BSA Free

Western Blot: SKA2 Antibody [NBP1-76312] - 3T3 cell lysate with SKA2 antibody at (A) 0.5 and (B) 1 ug/mL.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SKA2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SKA2 Antibody - BSA Free (NBP1-76312) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human SKA2. The immunogen is located within the first 50 amino acids of SKA2. Amino Acid Squence: YRLEYEIKTNHPDSA
• Predicted Species: Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SKA2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 0.5 - 1 ug/ml
• Control Peptide:

  SKA2 Peptide (NBP1-76312PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SKA2 Antibody - BSA Free

• FAM33A
• family with sequence similarity 33, member A
• FLJ12758
• MGC110975
• spindle and kinetochore associated complex subunit 2
• spindle and kinetochore-associated protein 2
• spindle and KT (kinetochore) associated 2

Background

Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SKA2
• Uniprot:
  • NP_872426(Human)