SKA1 Antibody

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP2-20376] - Paraffin-embedded rat testis. C18orf24 antibody diluted at 1:500

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP2-20376] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: SKA1 Antibody [NBP2-20376] - Paraffin-embedded mouse liver. C18orf24 antibody diluted at 1:500.

• Reactivity: Hu, Mu, Rt, RM
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SKA1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SKA1. The exact sequence is proprietary.
• Localization: Spindle; Kinetochore
• Predicted Species: Rhesus Macaque (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SKA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SKA1 Antibody

• C18orf24
• chromosome 18 open reading frame 24
• MGC10200
• spindle and kinetochore associated complex subunit 1
• spindle and kinetochore-associated protein 1
• spindle and KT (kinetochore) associated 1

Background

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SKA1
• Uniprot:
  • Q96BD8(Human)