SIX2 Antibodies
We offer SIX2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, RNA Inhibition, RNAi sequence position, Sandwich ELISA, Western Blot. Each SIX2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our SIX2 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the SIX2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our SIX2 polyclonal antibodies and browse our SIX2 monoclonal antibody catalog.
Alternate Names for SIX2 Antibodies
anti-SIX2 antibody, anti-homeobox protein SIX2 antibody, anti-sine oculis homeobox (Drosophila) homolog 2 antibody, anti-sine oculis homeobox homolog 2 (Drosophila) antibody, anti-Sine oculis homeobox homolog 2 antibody, anti-SIX homeobox 2 antibody
6 Results for "six2" in Primary Antibodies
![Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Western-Blot-H00010736-M01-img0008.jpg "Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 expression in transfected 293T cell line by SIX2 monoclonal antibody (M01), clone 3D7.Lane 1: SIX2 transfected lysate(32.3 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ and hFK serum-free cultures. Mouse embryonic kidney organ culture stained for Ncam1, Six2 and E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with and processed in ImageJ/Fiji software. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Immunocytochemistry-Immunofluorescence-H00010736-M01-img0010.jpg)
![Western Blot: SIX2 Antibody (3D7) [H00010736-M01] - Analysis of SIX2 over-expressed 293 cell line, cotransfected with SIX2 Validated Chimera RNAi ( Cat # H00010736-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SIX2 monoclonal antibody (M01), clone 3D7 (Cat # H00010736-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Western-Blot-H00010736-M01-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulation. Double labelling of sorted NCAM1+ cells for NCAM1 and SIX2: NCAM1 with DAPI (upper panel; red and blue channels), NCAM1 with SIX2 (lower panel; red and green channels), indicating both NCAM1+ SIX2+ (arrows) and NCAM1+ SIX2- (arrowheads) cells. Image collected and cropped by CiteAb from the following publication (//embomolmed.embopress.org/cgi/doi/10.1002/emmm.201201584), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-Immunocytochemistry-Immunofluorescence-H00010736-M01-img0011.jpg)
![ELISA: SIX2 Antibody (3D7) [H00010736-M01] - Detection limit for recombinant GST tagged SIX2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SIX2-Antibody-3D7-ELISA-H00010736-M01-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415485112.jpg)
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - Characterization of immunosorted NCAM1+ subpopulationA. RT-PCR analysis of gene expression in NCAM1 cell fractions. GAPDH was used as endogenous control & NCAM1− cell were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software & presented as average RQ ± SDEV of three replicates. ***p < 0.001, *p < 0.05 versus NCAM1−.B–D. Immunofluorescence staining of NCAM1+ & NCAM1− subpopulations for E-cadherin (E-cad) (B, red) vimentin (C, green) & SIX2 (D, green). Nuclei stained with Dapi (blue). (B–C) Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software, bar represents 200 μm. (D) Images were obtained using Zeiss LSM 510 confocal microscope, bar represents 50 μm.E. Fluorescent quantification of SIX2 immunostaining as represented in (D).F. Double labelling of sorted NCAM1+ cells for NCAM1 & SIX2: NCAM1 with DAPI (upper panel; red & blue channels), NCAM1 with SIX2 (lower panel; red & green channels), indicating both NCAM1+ SIX2+ (arrows) & NCAM1+ SIX2− (arrowheads) cells.G. Graph represents percentage of NCAM1+ SIX2+ cells & NCAM1+ SIX2− cells.H. Clonogenic efficiency of NCAM1+ cells sorted from hFK & cultured in SFM. Data are presented as average CE(%) ± SDEV. **p < 0.01 versus NCAM1−.I. Representative morphology of NCAM1+ & NCAM1− clones. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.J. Clonogenic capacity of hFK cells treated with IMGN901(ADC 55 nM), huN901 (Ab55 nM) or not treated (control). Data are presented as average CE(%) ± SDEV. ***p < 0.001, *p < 0.05 versus control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415482241.jpg)
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody (3D7) [H00010736-M01] - NCAM1 expression in mouse embryonic kidney organ & hFK serum-free culturesMouse embryonic kidney organ culture stained for Ncam1, Six2 & E-cad as indicated. An enlargement of the Ncam/Six2 signal is shown to emphasize the nuclear localization of Six2. White arrow illustrates the absence of Six2 signal in E-cad positive cells. An occurrence of Six2/E-cad positive cells is indicated with the asterisk. Images were obtained using Nikon A1R confocal microscope with & processed in ImageJ/Fiji software.Morphology of hFK cells cultured in SFM or SCM after 3 days (passage 0 Day 3—left panels) & towards confluence (14 days in SFM or 7 days in SCM—right panels). Distinct borders appear in SFM cultures (arrows) whereas cells with different morphology (arrows) are observed in SCM culture. Cells were observed using a Nikon Digital Sight camera attached to a Nikon Eclipse TS100 microscope.qRT-PCR analysis of gene expression in hFK cells cultured in SFM (three independent replicates). hPRT1 was used as endogenous control & SCM cells were used as the calibrator sample for RQ calculation (therefore = 1). Data were analysed using SDS 3.2 software.Representative FACS analysis of NCAM1 expression in hFK cells cultured in SFM at passage1. Data is presented in a histogram graph showing NCAM1 staining in blue & the isotype controls staining (negative control) in red.Immunofluorescence staining of NCAM1 (red) in total hFK cells cultured in SFM. Nuclei stained with Dapi (blue). Images were obtained using Olympus DP72 camera attached to Olympus BX51 fluorescence microscope & processed via cellSens standard software. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23996934), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00010736-m01_mouse-monoclonal-six2-antibody-3d7-310202415482281.jpg)
![Western Blot: SIX2 Antibody - BSA Free [NBP3-45348] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (SIX2 Antibody) at dilution of 1:600](http://images.novusbio.com/fullsize/nbp3-45348_rabbit-six2-pab-bsa-free-284202512371332.jpg "Western Blot: SIX2 Antibody - BSA Free [NBP3-45348] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (SIX2 Antibody) at dilution of 1:600")
![Immunoprecipitation: SIX2 Antibody - BSA Free [NBP3-45348] - IP Result of anti-SIX2 (IP:, 3ug; Detection: 1:500) with HEK-293 cells lysate 2700ug.](http://images.novusbio.com/fullsize/nbp3-45348_rabbit-six2-pab-bsa-free-284202512365443.jpg)
![Immunocytochemistry/ Immunofluorescence: SIX2 Antibody - BSA Free [NBP3-45348] - Immunofluorescent analysis of HEK293 cells using (SIX2 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)](http://images.novusbio.com/fullsize/nbp3-45348_rabbit-six2-pab-bsa-free-284202512582699.jpg)
![Western Blot: SIX2 Antibody [NBP2-54917] - Analysis in human cell line HeLa and human cell line A-549.](http://images.novusbio.com/fullsize/SIX2-Antibody-Western-Blot-NBP2-54917-img0002.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SIX2 Antibody [NBP2-54917] - Analysis in human cell line HeLa and human cell line A-549.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SIX2 Antibody [NBP2-54917] - Staining of human cell line U-2 OS shows localization to nuclear membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SIX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54917-img0001.jpg)
![Western Blot: SIX2 Antibody [NBP2-94801] - Western blot analysis of extracts of various cell lines, using SIX2 antibody (NBP2-94801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/SIX2-Antibody-Western-Blot-NBP2-94801-img0001.jpg "Western Blot: SIX2 Antibody [NBP2-94801] - Western blot analysis of extracts of various cell lines, using SIX2 antibody (NBP2-94801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: SIX2 Antibody [NBP2-94801] - Western blot analysis of extracts of Rat skeletal muscle cells, using SIX2 antibody (NBP2-94801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/SIX2 Antibody-Western Blot-NBP2-94801-img0002.jpg)
![Western Blot: SIX2 Antibody [NBP2-88276] - WB Suggested Anti-SIX2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/SIX2-Antibody-Western-Blot-NBP2-88276-img0001.jpg "Western Blot: SIX2 Antibody [NBP2-88276] - WB Suggested Anti-SIX2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle")
![Western Blot: SIX2 Antibody (1G11) [H00010736-M02] - Detection against Immunogen (57.75 KDa) .](http://images.novusbio.com/fullsize/SIX2-Antibody-1G11-Western-Blot-H00010736-M02-img0001.jpg "Western Blot: SIX2 Antibody (1G11) [H00010736-M02] - Detection against Immunogen (57.75 KDa) .")