Recombinant Human SIT1 His Protein

SDS-Page: Recombinant Human SIT1 Protein [NBP1-72451] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human SIT1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-196 of Human SIT1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MHLSQWTRGR SRSHPGQGRS GESVEEVPLY GNLHYLQTGR LSQDPEPDQQ DPTLGGPARA AEEVMCYTSL QLRPPQGRIP GPGTPVKYSE VVLDSEPKSQ ASGPEPELYA SVCAQTRRAR ASFPDQAYAN SQPAAS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SIT1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-PAGE
• Theoretical MW: 16.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SIT1 His Protein

• gp30/40
• MGC125908
• MGC125909
• RP11-331F9.5
• SHP2 interacting transmembrane adaptor
• SHP2-interacting transmembrane adapter protein
• SHP2-interacting transmembrane adaptor protein
• signaling threshold regulating transmembrane adaptor 1
• signaling threshold-regulating transmembrane adapter 1
• SIT1
• SITMGC125910
• suppression inducing transmembrane adaptor 1
• Suppression-inducing transmembrane adapter 1

Background

SIT1, also known as signaling threshold-regulating transmembrane adapter 1, negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. This protein involved in positive selection of T-cells. Recombinant human SIT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIT1