Sirtuin 3/SIRT3 Overexpression Lysate (Native)

Images

 
Western Blot: SIRT3 Overexpression Lysate (Adult Normal) [NBL1-15976] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRT3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Sirtuin 3/SIRT3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC217770 and based on accession number NM_001017524. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) (SIRT3), transcript variant 2, mRNA.
Gene
SIRT3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Sirtuin 3/SIRT3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Sirtuin 3/SIRT3 Overexpression Lysate (Native)

  • EC 3.5.1
  • EC 3.5.1.-
  • hSIRT3
  • mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
  • NAD-dependent deacetylase sirtuin-3, mitochondrial
  • SIR2L3
  • SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
  • sir2-like 3
  • SIR2-like protein 3
  • SIRT3
  • sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
  • Sirtuin 3
  • sirtuin type 3

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate splice site in the 5' end which causes the use of a downstream start codon, compared to variant 1. The resulting protein (isoform b) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Sirtuin 3/SIRT3 Lysate (NBL1-15976) (0)

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Bioinformatics

Gene Symbol SIRT3
Entrez