Sirtuin 3/SIRT3 Antibody (850911) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB
• Clone: 850911
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Sirtuin 3/SIRT3 Antibody (850911) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Sirtuin 3/SIRT3
  Ser101-Lys399
  Accession # Q9NTG7
• Specificity: Detects human Sirtuin 3/SIRT3 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Sirtuin 2 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sirtuin 3/SIRT3 Antibody (850911) [Alexa Fluor® 532]

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

Sirtuin 3 (SIRT3; SIR2-like protein 3) is a 28-30 kDa, NAD⁺-dependent class III member of the sirtuin protein family. It is widely expressed and found apparently in both the nucleus where it deacetylates histones, and in the mitochondrial matrix where it is involved in the regulation of both oxidative stress and the physiology associated with fasting or caloric restriction. Relative to the mitochondria, SIRT3 deacetylates multiple enzyme substrates including SOD2, IDH2, LCAD, OTC and HMGCS2, an action that results in enzyme activation. Human SIRT3 is 399 amino acids (aa) in length. It contains a mitochondrial targeting sequence within the first 100 aa, followed by a sirtuin-type deacetylase domain (aa 126-382) that contains an NAD binding segment (aa 145-165). SIRT3 is initially synthesized as a 42-44 kDa "precursor" in the cytosol. From here, it can translocate into mitochondria where it is cleaved between Arg99Arg100 to generate a 28-30 kDa short form. There is one potential alternative start site at Met143. Over aa 101-399, human and mouse SIRT3 share 84% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.