Sirtuin 3/SIRT3 Antibody (1A4)

Western Blot: Sirtuin 3/SIRT3 Antibody (1A4) [H00023410-M09] - Analysis of SIRT3 expression in transfected 293T cell line by SIRT3 monoclonal antibody (M09), clone 1A4. Lane 1: SIRT3 transfected lysate (Predicted MW: 28.6 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: Sirtuin 3/SIRT3 Antibody (1A4) [H00023410-M09] - Detection limit for recombinant GST tagged SIRT3 is 3 ng/ml as a capture antibody.

Product Discontinued

Sirtuin 3/SIRT3 Antibody (1A4) Summary

• Immunogen: SIRT3 (NP_036371.1, 297 a.a. ~ 399 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
• Specificity: SIRT3 - sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) (1A4)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Sirtuin 3/SIRT3 Antibody (1A4)

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT3
• Entrez:
  • 23410(Human)
• Uniprot:
  • NP_036371(Human)