SIRT6 Antibody (1D0L6)

Western Blot: SIRT6 Antibody (1D0L6) [NBP3-16229] - Western blot analysis of extracts of various cell lines, using SIRT6 Rabbit mAb (NBP3-16229) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

• Reactivity: Hu
• Applications: WB
• Clone: 1D0L6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SIRT6 Antibody (1D0L6) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 256-355 of human SIRT6 (Q8N6T7). GYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIRT6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SIRT6 Antibody (1D0L6)

• EC 3.5.1.-
• NAD-dependent deacetylase sirtuin-6
• SIR2L6sir2-related protein type 6
• SIR2-like protein 6
• sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
• sirtuin 6
• sirtuin type 6

Background

Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6)--a mouse Sir2 homolog--is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in the ageing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT6