SIRP alpha/CD172a Antibody (DH59B)

Flow Cytometry: SIRP alpha/CD172a Antibody (DH59B) [NBP2-61014] - Obtained with bovine leukocytes

• Reactivity: Hu, Po, Bv, Ca, Eq, Fe, Ll, Ma, Ma, Ma, Ma-Mn, Ma-WB, Sh
• Applications: Flow
• Clone: DH59B
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.

SIRP alpha/CD172a Antibody (DH59B) Summary

• Description: This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).
• Immunogen: Leukocytes from: Dog, bovine, horse
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRPA
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.09% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Notes

This monoclonal antibody is made in ascites form using mice. The ascites is collected and centrifuged to remove cells and large particles. The ascitic fluid is purified further by filtration through sequential filters with the last filter at 2 um. The concentration of the monoclonal antibody present in the ascites is determined with commercial radial immunodiffusion kits (1mg/ml).

Alternate Names for SIRP alpha/CD172a Antibody (DH59B)

• BIT
• BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs
• Brain Ig-like molecule with tyrosine-based activation motifs
• CD172 antigen-like family member A
• CD172a antigen
• CD172a
• Inhibitory receptor SHPS-1
• Macrophage fusion receptor
• MFR
• MFRtyrosine phosphatase SHP substrate 1
• MyD-1 antigen
• MYD1
• MYD-1
• p84
• protein tyrosine phosphatase, non-receptor type substrate 1
• PTPNS1
• SHP substrate 1
• SHPS1
• SHPS-1
• SHPS1CD172A
• signal-regulatory protein alpha
• Signal-regulatory protein alpha-1
• Signal-regulatory protein alpha-2
• Signal-regulatory protein alpha-3
• SIRP alpha
• SIRPA
• SIRPalpha
• Sirp-alpha-1
• SIRPalpha2
• Sirp-alpha-2
• Sirp-alpha-3
• SIRPtyrosine-protein phosphatase non-receptor type substrate 1

Background

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SIRP alpha/CD172a Antibody (NBP2-61014)(1)

Publication using NBP2-61014

• Xie S, Pan J, Zhang Q et al. Japanese Encephalitis Virus (JEV) NS1' Enhances the Viral Infection of Dendritic Cells (DCs) and Macrophages in Pig Tonsils Microbiology spectrum 2022-06-22 [PMID: 35730942] (Porcine)

Bioinformatics

• Gene Symbol: SIRPA