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SIM2 Antibody

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Western Blot: SIM2 Antibody [NBP2-95173] - Analysis of extracts of various cell lines, using SIM2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.
Western Blot: SIM2 Antibody [NBP2-95173] - Analysis of extracts of various cell lines, using SIM2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SIM2 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_033664.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIM2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SIM2 Antibody

  • human transcription factor SIM2, homolog of the Drosophila single-minded geneSIM110BHLHE15
  • short isoform
  • single-minded (Drosophila) homolog 2
  • single-minded homolog 2 (Drosophila)
  • transcription factor SIM2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Western Blot analysis with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124], theoretical molecular weight 86.6 kDa. Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1A or HIF-2A expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1A-shRNA or HIF-2A-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. HIF-1B expression was used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY licence.
Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and ARNT/HIF-1 beta antibody (H1beta234)[NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight for ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Co-localization of HIF-2alpha with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124]. ICC/IF detecting indicated proteins using antibodies labelled with Alexa Fluor 555 (red, pseudocolour) and Alexa Fluor 488 (green, pseudocolour). 'Merge' is the red image superimposed onto the green image of the co-stained nuclear proteins. 'Coloc' is the co-localization channel calculated using ImageJ plugin. White indicates pixels where both red and green signal is found (i.e. co-localization). 'Overlay' is the co-localization image superimposed onto the merged image. Inlay is the magnified region (white square). White arrows highlight regions of co-localization. Scale bar, 5 um. Abbreviations: RNAPII, RNA Polymerase II phospho-serine 5. Image collected and cropped by CiteAb from the following publication (http://rsob.royalsocietypublishing.org/lookup/doi/10.1098/rsob.160195), licensed under a CC-BY licence.
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(99 Publications)
Species: Bv, Ma, Hu, Mu, Pm, Rt, Sh
Applications: CHIP-SEQ, ChIP, GS, IB, ICC/IF, IHC, IHC-P, IP, WB
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Free
NB100-2289
Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-2289] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-AHR at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: AHR Antibody [NB100-2289] - An intracellular stain was performed on U-87 cells with AHR Antibody NB100-2289B (blue) and a matched isotype control (orange). Both antibodies were conjugated to Biotin. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Knockdown Validated: AHR Antibody [NB100-2289] - Activation of AHR/Nrf2 pathway by PFT-alpha. Knock-down efficiency of AHR siRNA as detected by qPCR (upper part) and western blot (lower part). Relative expression level is shown as fold change normalized to scramble siRNA. All values represent the mean +/- SD of two independent experiments performed in three replicates. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-020-58051-1), licensed under a CC-BY licence.
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Immunoprecipitation: SRM Antibody [NBP2-97669] - SRM was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-SRM rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SRM rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa
Western Blot: SRM Antibody [NBP2-97669] - Anti-SRM rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa
Immunohistochemistry-Paraffin: SRM Antibody [NBP2-97669] - Immunochemical staining of human SRM in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Species: Hu
Applications: IHC, IHC-P, IP, WB
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NBP2-55947
Immunocytochemistry/Immunofluorescence: SRMS Antibody [NBP2-55947] - Staining of human cell line MCF7 shows localization to cytosol & intermediate filaments.
Species: Hu
Applications: ICC/IF
BMAL1 Antibody
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Flow (Intracellular): BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on HeLa cells with NB100-2288AF647(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Immunohistochemistry: BMAL1 Antibody [NB100-2288] - Analysis of BMAL1 in mouse cerebral cortex (left: wt, right: Bmal1 KO) using anti-BMAL1 antibody. Image from verified customer review.
Flow Cytometry: BMAL1 Antibody [NB100-2288] - An intracellular stain was performed on Jurkat cells with NB100-2288 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.
(1 Review)
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Species: Hu, Mu, Pm, Rt
Applications: ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Naive CD4 T cells from WT, VHL-deficient (Vhl KO), or HIF-1 alpha-deficient (HIF-1 alpha KO) mice were differentiated under IL-22-skewing conditions for a total of 60 h. Some cells remained at normoxia for the duration of the culture (N); others were at normoxia for 35 h and then hypoxia (1% O2) for 24 h (H). At 60 h, nuclear extracts were harvested, and HIF-1 alpha and Lamin B1 levels were analyzed by Western blot. Image from verified customer review.
Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor(R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in H929 cells using HIF-1 alpha antibody. Flow cytometry image submitted by a verified customer review.
Flow Cytometry: HIF-1 alpha Antibody (H1alpha67) [NB100-105] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5 x 10^6) were stained with Alexa Fluor 488(R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine.
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Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp
Applications: ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB
SIM1 Antibody
NBP2-84260
Western Blot: SIM1 Antibody [NBP2-84260] - Host: Rabbit. Target Name: SIM1. Sample Tissue: Mouse Mouse Brain. Antibody Dilution: 1ug/ml
Immunohistochemistry: SIM1 Antibody [NBP2-84260] - Mouse embryonic spinal cord. Embryonic day 15.
Species: Mu
Applications: IHC, IHC-P, WB
Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Staining of human liver shows low expression as expected.
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Phenylalanine
Hydroxylase Antibody
NBP2-48615
Western Blot: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis using Anti-PAH antibody NBP2-48615 (A) shows similar pattern to independent antibody NBP1-80917 (B).
Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP2-48615] - Analysis in human liver and pancreas tissues. Corresponding Phenylalanine Hydroxylase RNA-seq data are presented for the same tissues.
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Species: Hu
Applications: IHC, IHC-P, WB
BBS9 Antibody
NBP1-88927
Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
(3 Publications)
Species: Hu, Mu
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OASIS/CREB3L1
Antibody
NB600-799
Western Blot: OASIS/CREB3L1 Antibody [NB600-799] - CREB3L1 Antibody [NB600-799] - Approx 60kDa band observed in lysates of cell line A549 (calculated MW of 57.0kDa according to NP_443086.1).
Immunohistochemistry-Paraffin: OASIS/CREB3L1 Antibody [NB600-799] - staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: OASIS/CREB3L1 Antibody [NB600-799] - staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(1 Review)
(1 Publication)
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
PER1 Antibody
NBP2-24589
Immunohistochemistry-Paraffin: PER1 Antibody [NBP2-24589] - Human brain tissue using an isotype control (top) and NBP2-24589 (bottom) at 5 ug/ml.
Western Blot: PER1 Antibody [NBP2-24589] - Analysis of PER1 in HEPG2 cell lysates in the 1) absence and 2) presence of immunizing peptide using NBP2-24589. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
(1 Review)
(2 Publications)
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: IB, IHC, IHC-P, KO, WB
SUMO2 Antibody
(SUMO2/1199) - Azide ...
NBP2-47710
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710] - Human Tonsil stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710] - Rat Heart stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Immunohistochemistry-Paraffin: SUMO2 Antibody (SUMO2/1199) - Azide and BSA Free [NBP2-47710] - Rat Ovary stained with SUMO-2 Monoclonal Antibody (SUMO2/1199)
Species: Hu, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P

Trappin-2/Elafin/Skalp
DY1747
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(14 Publications)
Species: Hu
Applications: ELISA
EGFR Antibody
[Unconjugated]
AF231
EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA‑MB‑231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EGFR at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Kallikrein 3/PSA
[HRP]
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(1 Review)
(16 Publications)
Species: Hu
Applications: ELISA
ASC/TMS1 Antibody
- BSA Free
NBP1-78977
Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - NLRP3 activation was evaluated by visualizing the co-localization of NLRP3 and apoptosis-associated speck-like protein (ASC) using immunocytochemistry (A). PLoS One. 2020 Jun 17;15(6):e0234039. doi: 10.1371/journal.pone.0234039. (Catalog # NBP1-78977AF647)
Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Rats were infected by S. pneumoniae or given PBS sham control. 21.5 umoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 minutes post-inoculation, and heart tissues were harvested 24 hours later. Heart sections were co-stained with anti-ASC (brown) and haematoxylin (blue). Negative control was stained with secondary antibody alone. Images are representative of a random selection of at least 3 sections of N = 6. PLoS One. 2015 Oct 8;10(10):e0139416. doi: 10.1371/journal.pone.0139416.
(2 Reviews)
(28 Publications)
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IM, IP, Simple Western, WB
Recombinant Human
PRH2 GST (N-Term) Pro ...
H00005555-P01
SDS-Page: Recombinant Human PRH2 Protein [H00005555-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Thymidylate
Synthase Antibody (SP
NBP2-34390
Flow (Intracellular): Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - An intracellular stain was performed on HEK293 cells with Thymidylate Synthase Antibody [SPM453] NBP2-34441PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (SPM453).
Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Thymidylate Synthase Mouse Monoclonal Antibody (SPM453).
(1 Review)
Species: Hu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, WB
SIM2 Antibody
NBP2-95173
SIM2 Antibody
Species: Hu
Applications: WB, IHC, IHC-P

Publications for SIM2 Antibody (NBP2-95173) (0)

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Bioinformatics

Gene Symbol SIM2