Siglec-9 Antibody [Biotin]

Human peripheral blood monocytes were stained with Goat Anti-Human Siglec‑9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1139, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Siglec-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Siglec-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, Flow, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Siglec-9 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec-9
  Gln18-Gly348
  Accession # Q9Y336
• Specificity: Detects human Siglec‑9 in direct ELISAs and Western blots. In direct ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) Siglec-2, rhSiglec-3, rhSiglec-5, rhSiglec-7, and rhSiglec-10 is observed. In Western blots, approximately 25% cross‑reactivity with rhSiglec-7 is observed, approximately 5% cross‑reactivity with rhSiglec-3 and rhSiglec-5 is observed, and less than 1% cross‑reactivity with rhSiglec-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human Siglec-9 Antibody (Catalog # MAB1139)
  ELISA Detection: Human Siglec-9 Biotinylated Antibody (Catalog # BAF1139)
  Standard:Recombinant Human Siglec-9 Fc Chimera (Catalog # 1139-SL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-9 Antibody [Biotin]

• CD329
• CDw329
• FOAP-9
• OBBP-Like
• Protein FOAP-9
• sialic acid binding Ig-like lectin 9
• sialic acid-binding Ig-like lectin 9
• Siglec9
• Siglec-9

Background

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-9 Antibody (BAF1139)(5)

Publications using BAF1139

• OS Adeniji, L Kuri-Cerva, C Yu, Z Xu, M Ho, GM Chew, C Shikuma, C Tomescu, AF George, NR Roan, LC Ndhlovu, Q Liu, K Muthumani, DB Weiner, MR Betts, H Xiao, M Abdel-Mohs Siglec-9 defines and restrains a natural killer subpopulation highly cytotoxic to HIV-infected cells PloS Pathogens, 2021-11-11;17(11):e1010034. 2021-11-11 [PMID: 34762717] (Depletion, Human)
• A Lizcano, I Secundino, S Döhrmann, R Corriden, C Rohena, S Diaz, P Ghosh, L Deng, V Nizet, A Varki Erythrocyte sialoglycoproteins engage Siglec-9 on neutrophils to suppress activation Blood, 2017-04-17;0(0):. 4/17/2017 [PMID: 28416510]
• Ismael Secundino, Anel Lizcano, K. Markus Roupé, Xiaoxia Wang, Jason N. Cole, Joshua Olson et al. Host and pathogen hyaluronan signal through human siglec-9 to suppress neutrophil activation Journal of Molecular Medicine 2/1/2016 [PMID: 26411873]
• Ismael Secundino, Anel Lizcano, K. Markus Roupé, Xiaoxia Wang, Jason N. Cole, Joshua Olson et al. Host and pathogen hyaluronan signal through human siglec-9 to suppress neutrophil activation Journal of Molecular Medicine 2016-02-01 [PMID: 26411873] (Immunoprecipitation, Western Blot, Neutralization, Human)
• Zou Z, Chastain A, Moir S Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids. PLoS ONE, 2011-09-08;6(9):e24559. 2011-09-08 [PMID: 21931755] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SIGLEC9
• Uniprot:
  • Q9Y336()