Siglec-9 Antibody [Unconjugated]

Siglec-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Siglec-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1139) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Siglec-9 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Siglec-9
  Gln18-Gly348
  Accession # Q9Y336
• Specificity: Detects human Siglec-9 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) Siglec-7 is observed, 5% cross-reactivity with rhSiglec-3 and rhSiglec-5 is observed, and less than 1% cross-reactivity with rhSiglec-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SIGLEC9
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Siglec-9 Antibody [Unconjugated]

• CD329
• CDw329
• FOAP-9
• OBBP-Like
• Protein FOAP-9
• sialic acid binding Ig-like lectin 9
• sialic acid-binding Ig-like lectin 9
• Siglec9
• Siglec-9

Background

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain, which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

1. Crocker, P.R. et al. (1998) Glycobiology 8:v.
2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.
6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Siglec-9 Antibody (AF1139)(4)

Publications using AF1139

• Z Zeng, M Li, M Wang, X Wu, Q Li, Q Ning, J Zhao, Y Xu, J Xie Increased expression of Siglec-9 in chronic obstructive pulmonary disease Sci Rep, 2017-08-31;7(1):10116. 2017-08-31 [PMID: 28860481] (IHC, Human)
• Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity. J Neurosci, 2015-02-11;35(6):2452-64. 2015-02-11 [PMID: 25673840] (Western Blot, Rat)
• Munetoshi Ando, Toru Shoji, Wenjie Tu, Hiroshi Higuchi, Ken-ichi Nishijima, Shinji Iijima Lectin-dependent localization of cell surface sialic acid-binding lectin Siglec-9 Cytotechnology 2015-08-01 [PMID: 24449467] (Immunocytochemistry, Mouse)
• Ando M, Tu W, Nishijima K, Iijima S Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs. Biochem. Biophys. Res. Commun., 2008-03-04;369(3):878-83. 2008-03-04 [PMID: 18325328] (Mouse)

Bioinformatics

• Gene Symbol: SIGLEC9
• Uniprot:
  • Q9Y336()