Siglec-9 Antibody (OTI1D9) - Azide and BSA Free

Western Blot: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Siglec-9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Siglec-9.

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Staining of paraffin-embedded Human liver tissue using anti-Siglec-9 mouse monoclonal antibody.

Western Blot: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Siglec-9 monoclonal antibody.

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Siglec-9 mouse monoclonal antibody.

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Siglec-9 mouse monoclonal antibody.

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Siglec-9 mouse monoclonal antibody.

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Staining of paraffin-embedded Human Kidney tissue using anti-Siglec-9 mouse monoclonal antibody.

Immunohistochemistry: Siglec-9 Antibody (OTI1D9) - Azide and BSA Free [NBP2-74189] - Staining of paraffin-embedded Human pancreas tissue using anti-Siglec-9 mouse monoclonal antibody.

• Reactivity: Hu, Ca, Pm
• Applications: WB, IHC
• Clone: OTI1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Siglec-9 Antibody (OTI1D9) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human SIGLEC9 (NP_055256) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIGLEC9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000-40000
• Theoretical MW: 50.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Siglec-9 Antibody (OTI1D9) - Azide and BSA Free

• CD329
• CDw329
• FOAP-9
• OBBP-Like
• Protein FOAP-9
• sialic acid binding Ig-like lectin 9
• sialic acid-binding Ig-like lectin 9
• Siglec9
• Siglec-9

Background

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIGLEC9