Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

Western Blot: Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free [NBP2-80948] - Analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.

Immunohistochemistry: Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free [NBP2-80948] - Human lung tissue stained with CD33 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 44M12D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free Summary

• Immunogen: Amino acids 42-159 of human CD33 were used as the immunogen for the antibody.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD33
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Western Blot 2-5 ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

• CD33 antigen (gp67)
• CD33 antigen
• CD33 molecule
• CD33
• FLJ00391
• gp67
• myeloid cell surface antigen CD33
• p67
• sialic acid binding Ig-like lectin 3
• Sialic acid-binding Ig-like lectin 3
• Siglec3
• Siglec-3
• SIGLEC3gp67

Background

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Siglec-3/CD33 Antibody (NBP2-80948) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Immunofluorescence - paraffin Human 03/25/2020

Summary

• Application: Other
• Sample Tested: Bone marrow (acute myeloid leukemia)
• Species: Human
• Lot: N/A

Comments

• Comments: pH 9 antigen retrieval

Bioinformatics

• Gene Symbol: CD33