SHMT1 Antibody

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Western Blot: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody NBP2-32173 used for WB at 1 ug/ml. Detection: ...read more
Immunohistochemistry: SHMT1 Antibody [NBP2-32173] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SHMT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Western Blot: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (50 ug) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 NBP2-32173 used for WB at 1 ug/ml. Detection: Chemiluminescence with ...read more
Immunohistochemistry: SHMT1 Antibody [NBP2-32173] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- SHMT1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: ...read more
Immunoprecipitation: SHMT1 Antibody [NBP2-32173] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody NBP2-32173 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SHMT1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 453 to 483 of human Serine Hydroxymethyltransferase 1 using the numbering given in entry NP_004160.3 (GeneID 6470).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SHMT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2500
  • ELISA 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Use in ELISA reported in scientific literature (PMID 27695002). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using
NBP2-32173 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SHMT1 Antibody

  • 14 kDa protein
  • CSHMT
  • cytoplasmic serine hydroxymethyltransferase
  • Glycine hydroxymethyltransferase
  • MGC15229
  • MGC24556
  • serine hydroxymethyltransferase 1 (soluble)
  • serine hydroxymethyltransferase, cytosolic
  • Serine methylase
  • SHMTEC 2.1.2.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SHMT1 Antibody (NBP2-32173)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.


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(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SHMT1
Uniprot